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Trichoderma isolates were collected from the rhizosphere and rhizoplane of Chickpea and Rice ranged from 5°C to 45°C screened for temperature tolerance. Among all the temperature tested at 30°C the isolates showed maximum average mycelial diameter of 8.81cm. Minimum average colony diameter of 0.87 cm was recorded at 400C. Simultaneously isolates Ta12 showed maximum average colony diameter of 6.36 cm, which did not differ significantly from Ta14 (6.30 cm) and Ta13 (6.16 cm). | Int.J.Curr.Microbiol.App.Sci 2020 9 3 1164-1171 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 3 2020 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2020.903.136 Isolation and Evaluation of Temperature Tolerant Trichoderma Manju Sharma Stanzin Idong Roopali Sharma and Priya Singh Department of Plant Pathology GB Pant University of Agriculture and Technology Pantnagar Uttarakhand India Corresponding author ABSTRACT Keywords Biocontrol agent Trichoderma Isolation Evaluation and Crop health management Article Info Accepted 05 February 2020 Available Online 10 March 2020 Trichoderma isolates were collected from the rhizosphere and rhizoplane of Chickpea and Rice ranged from 5 C to 45 C screened for temperature tolerance. Among all the temperature tested at 30 C the isolates showed maximum average mycelial diameter of 8.81cm. Minimum average colony diameter of 0.87 cm was recorded at 400C. Simultaneously isolates Ta12 showed maximum average colony diameter of 6.36 cm which did not differ significantly from Ta14 6.30 cm and Ta13 6.16 cm . It was interesting to note that Isolate Ta15 showed maximum colony diameter 2.60 cm at 400C while most of the other isolates were not able to grow at this temperature. Similarly maximum 266.06 mg average mycelial fresh weight of all the isolates were recorded at 30 C followed by 20 C 229.42 mg . Significantly maximum 143.50 mg fresh weight was recorded at 40 C by Isolate Ta15. Clearly 300C is the best temperature for Trichoderma to grow and reproduce efficiently using as biocontrol agent. Introduction The fungi belonging to Trichoderma spp. are in current use as Biocontrol agents due to their antagonistic potential against plant pathogens all over the world. The antifungal properties of Trichoderma are attributed to their ability to compete for nutrients secretions of hydrolytic enzymes and production of antibiotics. .
