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Sunflower is the fourth most important oilseed crop in the world after soybean, Rapeseedmustard and groundnut. It had 20 genera and 400 subspecies. Commercial cultivation of sunflower was started in India during 1972. In India, first ever sunflower hybrid developed by Seetharam (1980), the sunflower hybrids development is based on new sources of cytoplasmic male sterility (CMS). So far single source of cytoplasm PET-1 has been exploited extensively for hybrid (F1) production in sunflower. | Cytoplasmic diversity studies in sunflower (Helianthus annuus L.): A review