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The Staphylococcus aureus is a versatile opportunistic pathogen affecting both human and animals. The Genotyping of S. aureus isolates helps to understand the pathogen’s dissemination and epidemiological clusters, Hence, the present study designed to study the genotyping Staphylococcus aureus with RS-PCR from Chicken meat in Chennai City. In the present study, ribosomal spacer PCR (RS-PCR) was found to be showing polymorphism with varying band size and on software based dendrogram analysis. | Genotyping staphylococcus aureus isolates with RS-PCR from chicken meat in Chennai city