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Male sterility in vegetables is commercially utilized to develop their hybrids due to cost effective and easy mechanism to produce large-scale hybrid seeds commercially. Different types of male sterility, and three-line and two-line hybrid breeding approaches involving Cytoplasmic male stesility (CMS) and environmental sensitive genic male sterile (GMS) lines, are used for hybrid seed production. | Int.J.Curr.Microbiol.App.Sci 2020 9 2 51-65 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 2 2020 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2020.902.008 CMS Line in Carrot Chetna Chugh1 Sheshnath Mishra2 Manisha Mangal3 Shrawan Singh3 and Pritam Kalia3 1 Division of Biochemistry ICAR-Indian Agricultural Research Institute New Delhi-110012 India 2Shri Vaishnav Institute of Agriculture Science Shri Vaishnav Vidyapeeth Vishwavidyalaya Indore-453111 India 5Division of Vegetable Science ICAR-Indian Agricultural Research Institute New Delhi-110012 India Corresponding author ABSTRACT Keywords Hybrid carrot seed Cytoplosmic male sterility Maintainer Molecular Markers PCR. Article Info Accepted 05 January 2020 Available Online 10 February 2020 Male sterility in vegetables is commercially utilized to develop their hybrids due to cost effective and easy mechanism to produce large-scale hybrid seeds commercially. Different types of male sterility and three-line and two-line hybrid breeding approaches involving Cytoplasmic male stesility CMS and environmental sensitive genic male sterile GMS lines are used for hybrid seed production. In carrot which produces many but small sized flowers making hand-emasculation tedious and time consuming. Identification of male sterile and fertile counterpart of CMS lines is also not possible at early seedling stage by using test cross methods. Marker assisted selection for male sterile and fertile maintainer genes greatly enhance selection of A and B plants in breeding program. So molecular markers that can distinguish CMS status and fertile plants at an early seedling stage will be valuable tool in carrot breeding including CMS based hybrid seed programs in hybrid seed purity evaluation selection and management. Keeping these in views the present study was undertaken to characterize different sources of cytoplasmic male .
