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Fen liquor is typical of Chinese light-flavor liquor, which is fermented from sorghum with Fen-Daqu powder. Fen-Daqu is a saccharifying agent and fermentation starter obtained by natural solid-state fermentation under non-sterile conditions. The standard plate count and the online measurement methods were used to enumerate the surviving microorganisms, and measure the physiochemical in Fen-Daqu during the incubation. Total counts of mesophilic aerobic bacteria (30 °C and 55 °C), bacterial endospore (30 °C and 55 °C), lactic acid bacteria, enterobacteriaceae and fungi starting with minimum level around 106 , | Microbial and physiochemical changes during the incubation of fen-Daqu