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Present investigation was carried out to screen two promising sugarcane varieties CoN 13073 and CoN 09072 for salinity tolerance through callus culture. Various physiological and morphological parameters was studied, maximum shoot length was observed at 2.0 % NaCl concentration treated to callus at 65 days of culturing in variety CoN 13073, similar trend was observed for root length and leaf number per plant in variety CoN 13073 at 2.0 % NaCl concentration Very little differences observed in shoot: root ratio in variety CoN 09072, while there was much difference was observed in shoot : root ratio at various levels of NaCl in variety CoN 13073S. | In vitro response of promising sugarcane varieties for salinity tolerance through callus culture