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The results indicated that exposure to 10 and 30 µg L–1 Pb2+ significantly altered the activities of catalase, superoxide dismutase, and glutathione peroxidase in different tissues, while malondialdehyde content increased in a time- and concentration-dependent manner. Inhibition of AchE after chronic exposure also confirmed a generalized neurotoxic stress. | Turkish Journal of Biology Turk J Biol (2015) 39: 427-437 © TÜBİTAK doi:10.3906/biy-1410-3 http://journals.tubitak.gov.tr/biology/ Research Article Organ-specific antioxidant defenses and FT-IR spectroscopy of muscles in Crucian carp (Carassius auratus gibelio) exposed to environmental Pb2+ 1 1,2, 1 1 1 Sher Ali KHAN , Xiaoyu LIU *, Hong LI , Wenting FAN , Bakht Ramin SHAH , 1 1 1 3 Jingna LI , Lu ZHANG , Suyan CHEN , Sher Bahadar KHAN 1 College of Food Science & Technology, Huazhong Agricultural University, Wuhan, P.R. China 2 Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan, P.R. China 3 Key Laboratory of Agricultural Microbiology, Ministry of Education, Wuhan, P.R. China Received: 02.10.2014 Accepted/Published Online: 16.01.2015 Printed: 15.06.2015 Abstract: Being a nonessential element, lead (Pb2+) can be toxic in trace amounts for freshwater fish. The present study was designed to evaluate the induction of oxidative stress, tissue-specific antioxidant response, acetyl cholinesterase (AchE) activities, and biochemical content of muscle in Crucian carp (Carassius auratus gibelio) after 96 h of acute exposure and 21 days of chronic exposure to various environmental concentrations (5, 10, and 30 µg L–1) of waterborne Pb2+. The results indicated that exposure to 10 and 30 µg L–1 Pb2+ significantly altered the activities of catalase, superoxide dismutase, and glutathione peroxidase in different tissues, while malondialdehyde content increased in a time- and concentration-dependent manner. Inhibition of AchE after chronic exposure also confirmed a generalized neurotoxic stress. Fourier transform infrared spectroscopy revealed mild changes in the structure of proteins and lipids, whereas the integrated biomarker response indices signified the liver, kidneys, and gills as the responsive organs to environmental Pb2+ exposure. Key words: Crucian carp, heavy metal, Pb2+, fish, oxidative stress, Fourier transform infrared .
