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Lathyrus sativus L. (grass pea) is an important grain legume crop grown all over the world. It is very recalcitrant and difficult to regenerate under in vitro conditions. The present study determined the effect of preconditioning of cytokinins [10 and 20 mg L–1 thidiazuron (TDZ) and 2-isopentenyl adenine (2iP)] and postconditioning of dissimilar levels of TDZ-indole-3-butyric acid (IBA) and 2iP-IBA on in vitro shoot multiplication using embryonic node explants. | Turkish Journal of Biology Turk J Biol (2014) 38: 485-492 © TÜBİTAK doi:10.3906/biy-1312-94 http://journals.tubitak.gov.tr/biology/ Research Article Preconditioning effect of cytokinins on in vitro multiplication of embryonic node of grass pea (Lathyrus sativus L.) cultivar Gürbüz 1, 2 1 3 1 Surendra BARPETE *, Muhammad AASIM , Khalid Mahmood KHAWAR , Sancar Fatih ÖZCAN , Sebahattin ÖZCAN 1 Department of Field Crops, Faculty of Agriculture, Ankara University, Dışkapı, Ankara, Turkey 2 Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey 3 Central Agricultural Research Institute, Yenimahalle, Ankara, Turkey Received: 30.12.2013 Accepted: 28.03.2014 Published Online: 11.06.2014 Printed: 10.07.2014 Abstract: Lathyrus sativus L. (grass pea) is an important grain legume crop grown all over the world. It is very recalcitrant and difficult to regenerate under in vitro conditions. The present study determined the effect of preconditioning of cytokinins [10 and 20 mg L–1 thidiazuron (TDZ) and 2-isopentenyl adenine (2iP)] and postconditioning of dissimilar levels of TDZ-indole-3-butyric acid (IBA) and 2iP-IBA on in vitro shoot multiplication using embryonic node explants. The results showed that preconditioning followed by postconditioning on Murashige and Skoog (MS) medium with 0.25, 0.50, 0.75, and 1.0 mg L–1 2iP or TDZ combined with 0.10 mg L–1 IBA had positive effects on shoot multiplication. It seemed as if the TDZ-preconditioned and TDZ-IBA–postconditioned explants were more regenerative compared to 2iP-preconditioned or 2iP-IBA–postconditioned explants. Maximum shoot induction (100%) was achieved on MS medium containing 1 mg L–1 TDZ and 0.10 mg L–1 IBA, with a mean number of 31.10 shoots per explant. In comparison to the other cytokinin tested, 2iP-preconditioned and 2iP-IBA–postconditioned explants had longer shoots, with a mean shoot length of 8.10 cm. Root initiation was observed on all cultures 4 .
