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The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. | Turkish Journal of Biology Turk J Biol (2013) 37: 606-613 © TÜBİTAK doi:10.3906/biy-1301-16 http://journals.tubitak.gov.tr/biology/ Research Article Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat 1, 1 2 Aradhika TRIPATHI *, Rashmi AGGARWAL , Anita YADAV Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India 2 Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India 1 Received: 08.01.2013 Accepted: 16.04.2013 Published Online: 06.09.2013 Printed: 04.10.2013 Abstract: Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, 7 sets of primers produced remarkable induced transcripts. The highest up-regulated transcripts were observed in the resistant cultivar (HD 29), whereas the down-regulated transcripts were found in the susceptible (WL 711) cultivar. Several sets of primers were designed from the defenserelated gene sequences available in the GenBank database, and of these, 4 primer sets amplified the desired specific bands. Based on the putative functions, 3 defense-related genes, namely puroindoline protein PINB (AT_KB2), β-1,4-glucanase (AT_KB3), and chitinase (AT_KB7), and 1 housekeeping gene, actin (AT_KB1), were characterized. Key words: Defense-related genes, expression, DDRT-PCR, Tilletia indica, wheat 1. Introduction Wheat (Triticum aestivum L.) is the second
