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Different abiotic stresses recruit dedicated signaling and regulatory genes in plants. Genome-wide stress-specific biomarkers were investigated in tomatoes. Three major abiotic stresses were compared: Drought, heat, and salinity. For each stress type, 2 different tomato lines were included: Susceptible and tolerant. | Turkish Journal of Agriculture and Forestry http://journals.tubitak.gov.tr/agriculture/ Research Article Turk J Agric For (2014) 38: 700-715 © TÜBİTAK doi:10.3906/tar-1312-17 Transcriptomic analysis of tomato lines reveals putative stress-specific biomarkers 1,2, 1 2,3 Monther SADDER *, Abdullah ALSADON , Mahmoud WAHB-ALLAH Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia 2 Department of Horticulture and Crop Science, Faculty of Agriculture, The University of Jordan, Amman, Jordan 3 Vegetable Crops Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt 1 Received: 04.12.2013 Accepted: 31.03.2014 Published Online: 15.08.2014 Printed: 12.09.2014 Abstract: Different abiotic stresses recruit dedicated signaling and regulatory genes in plants. Genome-wide stress-specific biomarkers were investigated in tomatoes. Three major abiotic stresses were compared: drought, heat, and salinity. For each stress type, 2 different tomato lines were included: susceptible and tolerant. Gene expression was examined by hybridizing to an available tomato microarray. Several stress responsive genes were upregulated in tolerant as well as in susceptible lines for each stress. Comparative analysis of gene expression in response to stress (drought, heat, or salinity) resolved a number of common biomarkers, while other groups of putative biomarkers were associated with each abiotic stress. MYB transcription factors, SAUR family proteins, and NAC domain proteins were among the highly upregulated genes under drought, while both proteinase inhibitors and heat shock proteins were prominent in the heat-tolerant line. For salinity stress, the expression of phosphate starvation-induced proteins was observed. Putative abiotic stress biomarkers can be utilized in breeding programs to improve the selection process and to aid in gene stacking. Key words: Biomarkers, drought, heat, microarray, salinity,
