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Oxytropis glandulosa Turcz. (Fabaceae) is a rare perennial plant endemic to Buryatia (Russia). We sequenced three intergenic spacers (the psbA–trnH, trnL–trnF, and trnS–trnG) of chloroplast DNA (cpDNA) in specimens of four populations from two geographic regions (Barguzin and Yeravna depressions) within this species range. | Turkish Journal of Botany Turk J Bot (2018) 42: 38-50 © TÜBİTAK doi:10.3906/bot-1706-11 http://journals.tubitak.gov.tr/botany/ Research Article Plastid DNA variation of the endemic species Oxytropis glandulosa Turcz. (Fabaceae) 1, 1 1 2 3 2 Alla KHOLINA *, Marina KOZYRENKO , Elena ARTYUKOVA , Denis SANDANOV , Inessa SELYUTINA , Daba CHIMITOV 1 Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia 2 Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences, Ulan-Ude, Russia 3 Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia Received: 06.06.2017 Accepted/Published Online: 15.09.2017 Final Version: 11.01.2018 Abstract: Oxytropis glandulosa Turcz. (Fabaceae) is a rare perennial plant endemic to Buryatia (Russia). We sequenced three intergenic spacers (the psbA–trnH, trnL–trnF, and trnS–trnG) of chloroplast DNA (cpDNA) in specimens of four populations from two geographic regions (Barguzin and Yeravna depressions) within this species range. The levels of haplotype and nucleotide diversity varied in the range of 0.133–0.911 and 0.0002–0.0059, respectively. The highest values of these parameters are characteristic of populations located in the Barguzin depression. Variable sites detected within the intergenic spacers allowed the identification of eleven haplotypes; nine of them were found in the populations from the Barguzin depression and only two haplotypes were found in the populations from the Yeravna depression. No common haplotypes for all four populations were found. A high level of population differentiation (ΦST = 0.758, P 75%) and less than 25% of the total genetic variance was within populations. Overall differentiation among all populations was high and significant (ΦST = 0.75783, P < 0.0001; Table 3), while the level of the inferred gene flow was low (Nm = 0.25 .
