Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
This study aims to explore the role and the possible underlying molecular mechanism of SNF5 in the pathogenesis of rheumatoid arthritis (RA). MH7A cells were respectively transfected with pc-SNF5 (pcDNA3.1 containing the Brg1 coding sequence), short hairpin RNA against SNF5 (shSNF5), and their negative controls (pcDNA3.1 and shNC). | Turkish Journal of Biology Turk J Biol (2017) 41: 535-542 © TÜBİTAK doi:10.3906/biy-1610-40 http://journals.tubitak.gov.tr/biology/ Research Article Role of SNF5 in rheumatoid arthritis by upregulation of p16 and inactivation of JNK pathway 1, 1 2 1 Shupeng WU *, Jing WANG , Fang LI Department of Rheumatology and Immunology, Taian City Central Hospital, Taian, P.R. China 2 Department of Geriatrics, Taian City Central Hospital, Taian, P.R. China Received: 17.10.2016 Accepted/Published Online: 23.01.2017 Final Version: 14.06.2017 Abstract: This study aims to explore the role and the possible underlying molecular mechanism of SNF5 in the pathogenesis of rheumatoid arthritis (RA). MH7A cells were respectively transfected with pc-SNF5 (pcDNA3.1 containing the Brg1 coding sequence), short hairpin RNA against SNF5 (shSNF5), and their negative controls (pcDNA3.1 and shNC). The alterations of SNF5 expression were assessed by qRT-PCR and western blot analysis. MTT assay, flow cytometry, and western blot analysis were performed to evaluate proliferation, apoptosis, and expression levels of p16 and JNK pathway associated proteins, respectively. Finally, the effect of SNF5 was verified in fibroblast-like synoviocytes (FLSs) obtained from a rat model with adjuvant-induced arthritis. Results showed that the expression of SNF5 was increased in the pc-SNF5 group (P 0.05) between the two control groups (pcDNA3.1 and shNC) (Figures 1A and 1B). 3.2. Effects of SNF5 on cell proliferation When compared to the respective controls, cell viability analysis of MH7A cells in different groups revealed that cell viability was significantly reduced at 2 days, 3 days, and 4 days after transfection in the pc-SNF5 group (P 0.05) (Figures 3A and 3B). 3.4. Effects of SNF5 on the expressions of p16 and JNK pathway associated proteins Western blot analysis revealed a significant decrease (P < 0.05, P < 0.01, or P < 0.001) in the phosphorylated levels of the JNK pathway associated proteins,
