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In recent years, grafted cucumber seedling use has been rapidly increasing worldwide, especially for providing tolerance to stress conditions and positively affecting yield potential. However, graft incompatibility is still an important issue in grafted seedling production. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2018) 42: 124-135 © TÜBİTAK doi:10.3906/tar-1709-52 http://journals.tubitak.gov.tr/agriculture/ Research Article Use of phenotypic selection and hypocotyl properties as predictive selection criteria in pumpkin (Cucurbita moschata Duch.) rootstock lines used for grafted cucumber (Cucumis sativus L.) seedling cultivation 1, 2 3 3 Onur KARAAĞAÇ *, Ahmet BALKAYA , Münevver GÖÇMEN , İsmail ŞİMSEK , Dilek KANDEMİR 1 Black Sea Agricultural Research Institute, Samsun, Turkey 2 Department of Horticulture, Faculty of Agriculture, Ondokuz Mayıs University, Samsun, Turkey 3 Antalya Tarım Hybrid Seeds Inc., Antalya, Turkey 4 Samsun Vocational School, Ondokuz Mayıs University, Samsun, Turkey Received: 15.09.2017 Accepted/Published Online: 14.01.2018 4 Final Version: 26.04.2018 Abstract: In recent years, grafted cucumber seedling use has been rapidly increasing worldwide, especially for providing tolerance to stress conditions and positively affecting yield potential. However, graft incompatibility is still an important issue in grafted seedling production. Hypocotyl properties of rootstocks and scions are of great importance for ensuring graft compatibility. This study aimed to select superior rootstock genotypes based on certain selection criteria, such as hypocotyl morphology, grafted seedling visual evaluation, and graft compatibility properties, of 42 pumpkin (Cucurbita moschata) genotypes that are included in the cucumber rootstock breeding program and are resistant to Fusarium oxysporum f. sp. cucumerinum. Three C. moschata and three C. maxima × C. moschata rootstocks were used as control cultivars. All genotypes were grafted with the Gordion F1 cucumber cultivar using the splice grafting method. In the pumpkin rootstock genotypes, the cross-sectional area of the hypocotyls varied between 3.47 mm2 and 10.42 mm2, pith cavity area varied between 0.59 mm2 and 4.14 mm2, and pith cavity rate varied .