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European larch (Larix decidua) is naturally distributed at high elevations in Central Europe. Romanian populations of L. decidua are scattered in different areas of the country. In this study, we used 12 highly informative genomic simple sequence repeat (SSR) markers for genotyping seven populations from different areas of Romania. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2018) 42: 165-175 © TÜBİTAK doi:10.3906/tar-1801-41 http://journals.tubitak.gov.tr/agriculture/ Research Article Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania 1, 2, 3 3 1 Pietro GRAMAZIO *, Ioana M. PLESA *, Alina M. TRUTA , Adriana F. SESTRAS , Santiago VILANOVA , 4 4 5 1, 2 Mariola PLAZAS , Oscar VICENTE , Monica BOSCAIU , Jaime PROHENS **, Radu E. SESTRAS 1 Institute for Conservation and Improvement of Valencian Agrodiversity, Universitat Politècnica de València, Valencia, Spain 2 Department of Horticulture and Landscaping, Faculty of Horticulture, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania 3 Department of Forestry, Faculty of Horticulture, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania 4 Institute of Molecular and Cellular Plant Biology, (UPV-CSIC), Universitat Politècnica de València, Valencia, Spain 5 Mediterranean Agroforestry Institute, Universitat Politècnica de València, Valencia, Spain Received: 10.01.2018 Accepted/Published Online: 02.03.2018 Final Version: 29.05.2018 Abstract: European larch (Larix decidua) is naturally distributed at high elevations in Central Europe. Romanian populations of L. decidua are scattered in different areas of the country. In this study, we used 12 highly informative genomic simple sequence repeat (SSR) markers for genotyping seven populations from different areas of Romania. The SSR markers were highly variable, with up to 11 alleles per SSR locus and an average polymorphic information content of 0.713. High values of observed (Ho = 0.542) and expected (He = 0.738) heterozygosities were observed. Cluster analysis of populations did not group them according to geographical distance, but some clusters contained populations from a similar altitudinal range. The partition of .
