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Effect of cation concentrations (K+, Ca2+) and hormonal stimulation on sperm motility of common carp cyprinus carpio

This study aims to evaluate the effect of cation concentrations and hormonal stimulation on sperm motility of common carp. Experiments on the effect of cation concentrations use two types of cations in the following concentrations. | Journal of Fisheries science and Technology No.3 - 2016 EFFECT OF CATION CONCENTRATIONS (K+, Ca2+) AND HORMONAL STIMULATION ON SPERM MOTILITY OF COMMON CARP Cyprinus carpio Le Minh Hoang1, Pham Phuong Linh1 Received: 25/1/2016; Revised: 08/7/2016; Accepted: 26/9/2016 ABTRACT Semen quality assessment through sperm motility plays an important role in the production of artificial fish and contributes to the preservation of fish sperm more effectively. This study aims to evaluate the effect of cation concentrations and hormonal stimulation on sperm motility of common carp. Experiments on the effect of cation concentrations use two types of cations in the following concentrations: Ca2+ (0.5; 2.5; 5 mM) and K+ (5; 25; 50 mM), then use the best cation concentration to check the effects of the injection of the hormone on sperm motility. The results showed that the best cation concentration was 50 mM (K+) and 2.5 mM (Ca2+). These parameters before and after hormonal stimulation showed no difference. The results of this experiment can help us to create an environment for carp sperm activity based on the above parameters. Keywords: Carp, Cyprinus carpio, cation, hormone stimulation I. INTRODUCTION Spermatozoa of both marine and freshwater fish species are immotile in the testis and seminal plasma. Motility of spermatozoa occurs after they are released into surrounding aqueous environment during natural reproduction or into a diluents artificial reproduction (Islam and Akhter 2011). Spermatozoa motility is a prerequisite parameter in determining fish semen quality and fertilizing capacity (Le et al. 2011). However, spermatozoa motility is also influenced by several factors such as: temperature, dilution ratio, different diluents, ion concentration or osmolality (Le et al. 2011) and time after hormonal stimulation (Kowalski et al. 2012). Some studies have carried out with Persian surgeon - Acipenser persicus (Alavi et al. 2004), European perch - Perca fluviatilis (Alavi et al. .