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The European pine sawfly, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae), is a serious pest of pine plantations and ornamentals. Chemical pesticides used to control this pest have a detrimental effect on the environment. | Turk J Biol 25 (2001) 419-425 © TÜB‹TAK Viral Control of the European Pine Sawfly, Neodiprion sertifer (Geoffroy) in Turkey Mustafa YAMAN, Remziye NALÇACIO⁄LU, Zihni DEM‹RBA⁄ Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University, 61080, Trabzon - TURKEY Received: 23.10.2000 Abstract: The European pine sawfly, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae), is a serious pest of pine plantations and ornamentals. Chemical pesticides used to control this pest have a detrimental effect on the environment. The Neodiprion sertifer nuclear polyhedrosis virus (NsNPV) frequently causes considerable decreases in the populations but doses not harm other animals or plants. In this study, we tested five different concentrations of NsNPV against the larvae of N. sertifer and showed viral infection using Giemsa staining. When a viral concentration of 5x109 PIB/ml (Polyhedral Inclusion Body) was utilized, larval mortality started on the fourth day and reached 90.4% on the ninth day of the bioassay. However, lower viral concentrations, 5x109, 5x108 and 5x107 PIB/ml, showed the same mortality, but the infection periods occurred on different days. This study shows that NsNPV can be effectively used against N. sertifer as a safe biological control agent in Turkey. Key Words: Neodiprion sertifer, Nuclear Polyhedrosis Virus, Biological Control Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae)’in Viral Kontrolü Özet: K›rm›z›mt›rak sar› çal› antenli yaprakar›s› Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae) çam fidanl›klar›n›n önemli zararl›lar›ndan biridir. Bu zararl›y› kontrol etmek için kullan›lan kimyasal pestisidler çevrede zararl› etkilere sahiptir. N. sertifer nüklear polihedrozis virüsü (NsNPV) bu zararl›n›n populasyonunda önemli bir azalmaya neden olurken, çevredeki di¤er bitki ve hayvanlar› etkilememektedir. Bu makalede, N. sertifer larvalar›na karfl› NsNPV’nin farkl› konsantrasyonlar› test edildi ve viral enfeksiyon .
