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The growth of two robust algae strains Chlorella sp. and Scenedesmus sp. growing in Lithuanian lakes was investigated with the aim to obtain optimum conditions for biomass cultivation for biofuel production in the Lithuanian environment. | Aplinkos tyrimai, inžinerija ir vadyba, 2011. Nr. 3(57), P. 21 – 27 Environmental Research, Engineering and Management, 2011. No. 3(57), P. 21 – 27 http://erem.ktu.lt ISSN ISSN 1392-1649 (print) 2029-2139 (online) Cultivation of Microalgae Chlorella sp. and Scenedesmus sp. as a Potentional Biofuel Feedstock Violeta Makarevičienė1, Vaida Andrulevičiūtė1, Virginija Skorupskaitė1 and Jūratė Kasperovičienė2 1 Aleksandras Stulginskis University, Institute of Environment, Laboratory of Chemical and Biochemical Research for Environmental Technolog 2 Nature Research Centre, Institute of Botany, Laboratory of Hydrobotany (received in July, 2011, accepted in September, 2011) The growth of two robust algae strains Chlorella sp. and Scenedesmus sp. growing in Lithuanian lakes was investigated with the aim to obtain optimum conditions for biomass cultivation for biofuel production in the Lithuanian environment. Samples were taken from different nitrogen sources and of different concentrations, with addition of various concentrations of CO2 and in the presence of salt. The best biomass productivity was achieved using urea as a nitrogen source or modified growing medium BG11 with decreased concentration of NaNO3. The positive impact on the growth of biomass was achieved by aeration with CO2 (especially with concentration of 24%). Additional research into the removal of pollutants, such inorganic salts of nitrogen and phosphorus and organic materials from wastewater using microalgae has revealed good possibilities of using both algae strains in wastewater treatment plants. A content of oil in Chlorella sp. and Scenedesmus sp. has suggested their potential use as biodiesel feedstock. Key words: microalgae, biomass, growing conditions, CO2 fixation. 1. Introduction The study of different aspects related to the behavior of microalgae has received renewed interest due to the wide field of application of these microorganisms. Algae cultures have been principally developed as an
