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In this study, DNA barcoding approach using 18S rDNA and the mitochondrial cytochrome oxidase c subunit 1 was developed to assess the morphological based identification success of free-living marine nematode species of the families Desmodoridae and Microlaimidae from this mangrove. | HỘI NGHỊ KHOA HỌC TOÀN QUỐC VỀ SINH THÁI VÀ TÀI NGUYÊN SINH VẬT LẦN THỨ 6 MOLECULAR IDENTIFICATION OF FREE-LIVING MARINE NEMATODE SPECIES OF THE FAMILIES DESMODORIDAE AND MICROLAIMIDAE (NEMATODA: DESMODORIDA) FROM THE TIEN YEN MANGROVE, QUANG NINH PROVINCE NGUYEN THI XUAN PHUONG, NGUYEN DINH TU, NGUYEN VU THANH Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology Morphological identification of free-living nematodes is fragrantly difficult (Derycke et al., 2010) because of the morphological variation, the paucity of easily scorable diagnostic morphological characteristics or a complex evolution origin of these organisms (De Ley et al., 2005; Derycke et al., 2010). As an alternative to the morphological determination, molecular information is expected to provide better resolutions and typification of marine nematodes, especially molecular barcoding, a method using one or more parts of the genome as an identifying tag (Hebert et al., 2003). Molecular barcodes can be preferable to morphological identification because they allow the analysis of all stages of all species, leading to unequivocal “molecular species” assignation and identification of novel taxa (Jörger & Schrödl, 2013). The data can be used for downstream analyses of phylogeny, taxonomy, abundance and diversity estimation, and biomonitoring. Although the important roles of molecular information of free-living marine nematodes are undeniable, their molecular data are still limited. In Vietnam, there was only a molecular study of the family Comesomatide was published (Nguyen et al., 2013). In order to contribute to the molecular data of free-living marine nematodes communities in the mangroves of Vietnam, this study was implemented at the Tien Yen district mangrove which is under the administration of the Quang Ninh Province, Northeastern Vietnam. In this study, DNA barcoding approach using 18S rDNA and the mitochondrial cytochrome oxidase c subunit 1 was developed to .
