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In vitro antioxidant properties of 3 different extracts of black mulberry (Morus nigra L.) were investigated. Acidified methanol, acidified water, and non-acidified methanol/water solutions were used to prepare extracts. | Turk J Biol 35 (2011) 103-110 © TÜBİTAK doi:10.3906/biy-0904-22 Antioxidant properties of different extracts of black mulberry (Morus nigra L.) Türkan KUTLU1, Gökhan DURMAZ2, Burhan ATEŞ1, İsmet YILMAZ1, M. Şevket ÇETİN2 1Department of Chemistry , Faculty of Science and Arts, İnönü University, TR-44280 Malatya - TURKEY 2Department of Food Engineering, Faculty of Engineering, İnönü University, TR-44280 Malatya - TURKEY Received: 30.04.2009 Abstract: In vitro antioxidant properties of 3 different extracts of black mulberry (Morus nigra L.) were investigated. Acidified methanol, acidified water, and non-acidified methanol/water solutions were used to prepare extracts. Different solvents caused different protonation on black mulberry anthocyanin structures, which were predicted according to their UV-VIS spectrum. Extracts indicated 2 main peaks at about ~280 and ~520 nm with different peak areas in their UV-VIS spectrum. The rank of extracts’ total phenolic content and reducing power values were both found to be in correlation with their absorbance at ~280 nm. Meanwhile the same relationship was observed between DPPH scavenging activity and absorbance values at ~520 nm. Acidified extract of black mulberry was higher in β-carotene prevention and DPPH radical scavenging activity than non-acidified extract. However, non-acidified extract represented a higher reducing power and metal chelating activity, and a higher content of total phenolics. Key words: Antioxidant properties, black mulberry, phenolic, extract, metal chelating activity Farklı karadut (Morus nigra L.) ekstraktlarının antioksidan özellikleri Özet: Üç farklı karadut (Morus nigra L.) ekstraktının in vitro antioksidan özellikleri araştırılmıştır. Ekstraktları hazırlamak için asitlendirilmiş metanol, asitlendirilmiş su ve asitlendirilmemiş metanol/su karışımı kullanılmıştır. Farklı solventler karadut antosiyanın yapıları üzerinde UV-VIS spektrumlarından anlaşılan farklı protonlanmaya sebep olmuştur. .
