TAILIEUCHUNG - Báo cáo khoa học: Enzymatic characterization of starch synthase III from kidney bean (Phaseolus vulgaris L.)

In plants and green algae, several starch synthase isozymes are responsible for the elongation of glucan chains in the biosynthesis of amylose and amy-lopectin. Multiple starch synthase isozymes, which are classified into five major classes (granule-bound starch synthases, SSI, SSII, SSIII, and SSIV) according to their primary sequences, have distinct enzymatic properties. | ễFEBS Journal Enzymatic characterization of starch synthase III from kidney bean Phaseolus vulgaris L. Takeshi Senoura Ayako Asao Yoshinori Takashima Naoto Isono Shigeki Hamada Hiroyuki Ito and Hirokazu Matsui Division of Applied Bioscience Graduate Schoolof Agriculture Hokkaido University Sapporo Japan Keywords affinity gel electrophoresis carbohydrate-binding module kinetics Phaseolus vulgaris L. starch synthase III Correspondence H. Ito Division of Applied Bioscience Graduate Schoolof Agriculture Hokkaido University Sapporo 060-8589 Japan Fax Tel 81 11 706 2508 E-mail otih@ Database Sequence data for PvSSIII cDNA appear in the DDBJ Nucleotide Sequence Database under the accession number AB293998 Received 29 March 2007 revised 22 June 2007 accepted 10 July 2007 doi In plants and green algae several starch synthase isozymes are responsible for the elongation of glucan chains in the biosynthesis of amylose and amylopectin. Multiple starch synthase isozymes which are classified into five major classes granule-bound starch synthases SSI SSII SSIII and SSIV according to their primary sequences have distinct enzymatic properties. All the starch synthase isozymes consist of a transit peptide an N-terminal noncatalytic region N-domain and a C-terminal catalytic region C-domain . To elucidate the enzymatic properties of kidney bean Phaseo-lus vulgaris L. SSIII and the function of the N-domain of kidney bean SSIII three recombinant proteins were constructed putative mature recombinant SSIII recombinant kidney bean SSIII N-domain and recombinant kidney bean SSIII C-domain. Purified recombinant kidney bean SSIII displayed high specific activities for primers as compared to the other starch synthase isozymes from kidney bean. Kinetic analysis showed that the high specific activities of recombinant kidney bean SSIII are attributable to the high kcat values and that the Km values of recombinant kidney bean SSIII C-domain .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• Structural biochemistry
• bacteria
• chemical reaction
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine