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There is a persistent environmental concern that transgenic Bt-crops have indirect undesirable effect to natural and agroecosystem function. We investigated the effect of Btcotton (with Cry 1 Ac gene) on soil biology in Bt cotton growing soils of Perambalur district, Tamil Nadu under rainfed scenario. Soil samples randomly from ten Bt cotton growing fields were selected in each of the taluks of Perambalur district of TamilNadu region, India, where Bt-cotton has been growing at least for ten continuous years and side by side non-Bt cotton grown soils were also collected to compare the extent of adverse effect of Bt toxin, if any. Samples were analyzed for various soil biological indicators like microbial population, microbial respiration, Microbial Biomass Carbon (MBC), Microbial Biomass Nitrogen (MBN), and soil Dehydrogenase (DHA) activities. The soil biological indicators like microbial population, soil respiration, DHA, MBC and MBN were found to be comparitively higher in Btgrown soils than their non Bt counter parts over a period of 10 years. | Studies on the impact of growing transgenic cotton on soil health in Major Bt cotton growing areas of Tamil Nadu, India