TAILIEUCHUNG - Circulation of Japanese Encephalitis Virus in Pigs and Mosquito Vectors within Can Tho City, Vietnam

Circulation of Japanese Encephalitis Virus in Pigs and Mosquito Vectors within Can Tho City, Vietnam Japanese encephalitis (JE) is a zoonotic disease spread over large parts of Asia. It is one of the most important arboviral encephalitis in humans, with an estimated 10 million cases over the last 60 years, with 30% case fatality. Pigs and wading birds are amplifying hosts of the causative Japanese encephalitis virus (JEV), and do not display clinical signs, except for pregnant sows that may abort or have stillborn piglets. | 05 I NEGLECTED 7TLV9 I TROPICAL DISEASES OPEN 3 ACCESS Freely available online Circulation of Japanese Encephalitis Virus in Pigs and Mosquito Vectors within Can Tho City Vietnam Johanna F. Lindahl1 Karl Stahl2 Jan Chirico3 Sofia Boovist2 Ho Thi Viet Thu4 Ulf Magnusson1 . 1 Department of Clinical Sciences Division of Reproduction Swedish University of Agricultural Sciences Uppsala Sweden 2 Department of Biomedicine and Veterinary Public Health Swedish University of Agricultural Sciences Uppsala Sweden 3 Department of Virology Immunology and Parasitology National Veterinary Institute National Veterinary Institute SVA Uppsala Sweden 4 Department of Veterinary Medicine Can Tho University Can Tho City Vietnam Abstract Japanese encephalitis virus JEV is a mosquito-borne zoonotic flavivirus causing encephalitis in humans and reproductive disorder in pigs. JEV is present in large parts of Asia where urbanization is high. Households within and outside Can Tho city South Vietnam were selected to monitor circulation of JEV. A nested RT-PCR was established to detect the presence of JEV in mosquitoes whereas sera from pigs belonging to households within the province were analyzed for the presence of antibodies to JEV. A total of 7885 mosquitoes were collected and divided into 352 pools whereof seven were JEV-positive six of which were collected within the city. Fragments from four pools clustered with JEV genotype III and three with genotype I. Of the 43 pigs sampled inside the city 100 had JEV antibodies. Our study demonstrates exposure to JEV in pigs and co-circulation of JEV genotype I and III in mosquitoes within an urban environment in South Vietnam. Thus although JEV has mainly been considered a rural disease the potential for transmission in urban areas cannot be ignored. Citation Lindahl JF Stahl K Chirico J Boqvist S Thu HTV et al. 2013 Circulation of Japanese Encephalitis Virus in Pigs and Mosquito Vectors within Can Tho City Vietnam. PLoS Negl Trop Dis 7 4 .

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