TAILIEUCHUNG - Báo cáo Y học: The structure of the O-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of Hafnia alvei that has characteristics of a new species under the genus Escherichia

The structure of the O-polysaccharide of the lipopolysaccharide from a diarrheal strain isolated in Bangladesh was studied with sugar, and methylation analysis, NMR spectroscopy, mass spectrometry and partial acid hydrolysis. The strain was first designated as Hafnia alvei, but later found to be a possible new species in the genus Escherichia. Two different polysaccharides were detected, a major and a minor one. The structure of the major polysaccharide is given below, while the structure of the minor one was not investigated. . | Eur. J. Biochem. 269 3289-3295 2002 FEBS 2002 doi The structure of the O-chain of the lipopolysaccharide of a prototypal diarrheagenic strain of Hafnia alvei that has characteristics of a new species under the genus Escherichia Reine Eserstam1 Thushari P. Rajaguru1 2 Per-Erik Jansson1 Andrej Weintraub3 and M. John Albert4 1 Clinical Research Center Analytical unit Karolinska Institute Huddinge Hospital Huddinge Sweden 2Department of Chemistry University of Peradeniya Peradeniya Sri Lanka 3Karolinska Institute Department of Microbiology Pathology and Immunology Division of Clinical Bacteriology Huddinge University Hospital Sweden 4Department of Microbiology Faculty of Medicine Kuwait University Safat Kuwait The structure of the O-polysaccharide of the lipopolysaccharide from a diarrheal strain isolated in Bangladesh was studied with sugar and methylation analysis NMR spectroscopy mass spectrometry and partial acid hydrolysis. The strain was first designated as Hafnia alvei but later found to be a possible new species in the genus Escherichia. Two different polysaccharides were detected a major and a minor one. Tie ttnicture of the major polysaccharide is g--ven below while the structure of the minor one was not investigated. The 111 6 ff the repeating unit was esta lj-lished as 6 -P-D-Galf- 1 3 -P-D-GalpNAc- 1 3 -P-D-Galp- 1 6 T 2 a-NeuAc The structure does not resemble any of the previously investigated lipopolysaccharide O-chains from Escherichia coli or H. alvei but could fit in either group based on types of sugar residues and acidity. Phenotypic microbiological studies cannot definitely assign it to either species of the two genera. Genetic hybridization studies indicate that the Bangladeshi isolates may require a new species designation under the genus Escherichia. Keywords lipopolysaccharide Escherichia Hafnia alvei diarrhea neuraminic acid. Hafnia alvei is a Gram negative bacterium and a member of the family .

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