TAILIEUCHUNG - Báo cáo Y học: Atlantic salmon possess three mitogen activated protein kinase kinase 6 paralogs responding differently to stress

Mitogen activated protein kinase kinase (MKK) 3 and 6 are the main p38 mitogen-activated protein kinase activators in mammals. In the present study, three Atlantic salmon MKK6 orthologs were identified. The deduced amino acid sequences of the salmon MKK6 proteins were highly similar to mammalian MKK6 sequences, and they were ubiquitously expressed. All three were shown to be upstream activators of salmon p38. | ỊFEBS Journal Atlantic salmon possess three mitogen activated protein kinase kinase 6 paralogs responding differently to stress Tom E. Hansen1 2 Pal Puntervoll3 Ole Morten Seternes4 and Jorunn B. J0rgensen1 2 1 Department of Marine Biotechnology Norwegian College of Fishery Science University of Tromso Norway 2 The Norwegian StructuralBiology Centre NorStruct University of Tromso Norway 3 ComputationalBiology Unit Bergen Centre for ComputationalScience Norway 4 Department of Pharmacology Institute of MedicalBiology University of Tromso Norway Keywords MAP kinase MKK3 MKK6 p38 salmon Correspondence J. B. Jorgensen Department of Marine Biotechnology Norwegian College of Fishery Science University of Tromso N-9037 Tromso Norway Fax 47 77 64 60 20 Tel 47 77 64 67 16 E-mail Received 8 May 2008 revised 28 July 2008 accepted 4 August 2008 doi Mitogen activated protein kinase kinase MKK 3 and 6 are the main p38 mitogen-activated protein kinase activators in mammals. In the present study three Atlantic salmon MKK6 orthologs were identified. The deduced amino acid sequences of the salmon MKK6 proteins were highly similar to mammalian MKK6 sequences and they were ubiquitously expressed. All three were shown to be upstream activators of salmon p38. In cells exposed to sorbitol sodium arsenite and UV radiation the different salmon MKK6s were shown to be selectively activated. Thus our results suggest a specific function of the three salmon MKK6s depending on which stress stimuli the cells are exposed to. Phylogenetic analysis of MKK6 and MKK3 sequences from different species indicate that salmon is unique in having three MKK6 gene copies whereas other fish species possess one or two MKK6 genes. Interestingly in contrast to mammals fish do not have an MKK3 gene. We propose that two major duplication events have occurred for the ancestral MKK3 6 gene one in tetrapods yielding MKK3 and MKK6 and another one in fish .

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