TAILIEUCHUNG - Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum

A novel lyase, combining hydratase and aldolase activity, that converts citral intomethylheptenone and acetaldehyde, was purified from spores ofPenicillium , citral lyase activitywas induced 118-fold by incubating nongerminating spores with the substrate, citral. This cofactor independent hydratase/aldolase, was purified and found tobe amonomeric enzymeof 31 lyasehas aKmof aVmaxof UÆmg )1 . | Eur. J. Biochem. 269 5903-5910 2002 FEBS 2002 doi A novel inducible citral lyase purified from spores of Penicillium digitatum Wout A. M. Wolken1 Willem J. V. Van Loo1 Johannes Tramper2 and Mariet J. van der Werf1 3 1 Division of Industrial Microbiology Department of Food Technology and Nutritional Sciences Wageningen University the Netherlands 2Department of Food Technology and Nutritional Sciences Wageningen University Wageningen the Netherlands 3Department of Applied Microbiology and Gene Technology TNO Food Zeist the Netherlands A novel lyase combining hydratase and aldolase activity that converts citral into methylheptenone and acetaldehyde was purified from spores of Penicillium digitatum. Remarkably citral lyase activity was induced 118-fold by incubating nongerminating spores with the substrate citral. This cofactor independent hydratase aldolase was purified and found to be a monomeric enzyme of 31 kDa. Citral lyase has a Km of mM and a Vmax of U-mg-1. Enzyme activity was optimal at 20 C and pH . The enzyme has a strong preference for the trans isomer of citral geranial . Citral lyase also converts other a b-unsaturated aldehydes farnesal methyl-crotonaldehyde decenal and cinnemalde-hyde . Keywords hydratase aldolase induction a b-unsaturated aldehydes spores Penicillium digitatum. The linear monoterpene citral was originally reported to occur in lemongrass accounting for up to 75 of the oil. Citral was then also found in several other plant oils . in lemon and lime oil. Commercial citral is obtained by isolating it from citral-containing essential oils or by chemical synthesis from b-pinene or isoprene 1 . Citral is a mixture of the cis- and trans-isomers of 3 7-dimethyl-2 6-octadiene-1-al referred to as neral and geranial respectively. Commercial citral typically contains 60 geranial and 40 neral. Citral is widely used in the flavour and fragrance industry its application ranges from meat products to hard

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