TAILIEUCHUNG - Báo cáo Y học: Stimulated biosynthesis of flavins in Photobacterium phosphoreum IFO 13896 and the presence of complete rib operons in two species of luminous bacteria Sabu Kasai and Takumi Sumimoto

PhotobacteriumphosphoreumIFO13896 emits light strongly when cultured in medium containing 3% NaCl, but only weakly in medium containing 1% is known that dim or dark mutants appear frequently and spontaneously from this parent confirm that riboflavin biosyn-thesis is stimulatedwhen theluxoperon is active, the amount of light emitted and flavins synthesized under strongly or weakly light emitting conditions was determined. | Eur. J. Biochem. 269 5851-5860 2002 FEBS 2002 doi Stimulated biosynthesis of flavins in Photobacterium phosphoreum IFO 13896 and the presence of complete rib operons in two species of luminous bacteria Sabu Kasai and Takumi Sumimoto Department of Bioapplied Chemistry Faculty of Engineering Osaka City University Japan Photobacteriumphosphoreum IFO 13896 emits light strongly when cultured in medium containing 3 NaCl but only weakly in medium containing 1 NaCl. It is known that dim or dark mutants appear frequently and spontaneously from this parent strain. To confimi that riboflavin tjksyni-thesis is stimulated when the lux operon is active the amount of light emitted and flavins synthesized under strongly or weakly light emitting conditions was determined. hl oomparison with the parent strain cultured in 3 NaCl the same strain cultured in 1 NaCl emitted 1 36 the light and produced 1 4 the flavins while three dim or dark mutants M1 M2 and M3 cultured in 3 NaCl emitted almost no light 1 58 the light and 1 10 the light and produced 1 8 1 5 and 1 3 the amount of flavins respectively. From these resuks we deduced that the genes for riboflavin synthesis rib genes are organized in an operon in this strain. In P. phosphoreum NCMB 844 it has been reported that a rib gene cluster is present just downstream of the lux operon. However among rib genes the gene for pyrimidine deaminase pyrimidine reductase ribD was not found in this cluster. Bccau a complete rib operon seems to be necessary for efficient regulation at the transcriptional level we expected ribD to be present downstream of this cluster and sequenced this region using SUGDAT Sequencing Using Genomic DNA As a Template. We coslld not find this g t n e bull ICalnd a gene for hybrid-cluster protein prismane protein . To find ribD in a different region a partial ribD sequence was amplified and sequenced using a PCR-based method and subsequently the genomic DNA was sequenced in both .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• biological activities
• environmental medicine
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies