TAILIEUCHUNG - Application of Polymerase Chain Reaction for High Sensitivity Detection of Roundup Ready™ Soybean Seeds and Grains in Varietal Mixtures

Strong increase in the production of genetically modified organisms (GMOs) observed over the years has led to a consolidation of transgenic seed industries worldwide. The dichotomy between the evaluated risk and the perceived risk of transgenic use has defined their level of acceptability among different global societies. GMOs have been widely applied to agricultural commodities, among them the Roundup Ready™ (RR™) soybean line GTS 40-3-2 has become the most prevalent transgenic crop in the world. This variety was developed to confer plant tolerance against glyphosate-based agricultural herbicide Roundup Ready™. Issues related to detection and traceability of GMOs have gained worldwide interest due to their increasing global diffusion and. | . PINTO et al. PCR Detection of RR Soybean Seeds in Varietal Mixtures Food Technol. Biotechnol. 49 3 277-285 2011 277 ISSN 1330-9862 FTB-2757 original scientific paper Application of Polymerase Chain Reaction for High Sensitivity Detection of Roundup Ready Soybean Seeds and Grains in Varietal Mixtures Giovana Boff Araujo Pinto1 Marcelo Silva2 Ralf Greiner1 Uschi Konietzny1 Carlos Ricardo Soccol1 Michele Rigon Spier1 Marco Aurélio da Silva Carvalho Filho3 Ashok Pandey4 and Vanete Thomaz-Soccol1 3 Graduation Program in Biotechnological Process Bioprocess Engineering and Biotechnology Division Federal University of Parana UFPR . Box 19011 CEP 81531-970 Curitiba PR Brazil 2Agriculture and Supply Secretary of Parana State SEAB Funcionários 1559 CEP 80035-050 Curitiba PR Brazil 3Positivo University UP Master of Industrial Biotechnology Rua Prof. Pedro V. Parigot de Souza 5300 CEP 81280-330 Curitiba PR Brazil 4Biotechnology Division National Institute for Interdisciplinary Science and Technology NIIST CSIR 695 019 Trivandrum India Received September 16 2010 Accepted February 7 2011 Summary Strong increase in the production of genetically modified organisms GMOs observed over the years has led to a consolidation of transgenic seed industries worldwide. The dichotomy between the evaluated risk and the perceived risk of transgenic use has defined their level of acceptability among different global societies. GMOs have been widely applied to agricultural commodities among them the Roundup Ready RR soybean line GTS 40-3-2 has become the most prevalent transgenic crop in the world. This variety was developed to confer plant tolerance against glyphosate-based agricultural herbicide Roundup Ready . Issues related to detection and traceability of GMOs have gained worldwide interest due to their increasing global diffusion and the related socioeconomic and health implications. Also due to the widespread use of GMOs in food production labelling regulations have been .

• Báo cáo khoa học
• certification of conventional soybean seeds and grains
• detection of transgenic soybean
• polymerase chain reaction (PCR)
• Roundup Ready™ (RR™) soybean