TAILIEUCHUNG - Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits

A number of findings have suggested the involvement of protein phosphorylation in the regulation of the epithelial Na + channel (ENaC).Arecent studyhas demonstrated that the C tails of thebandcsubunits of ENaC are subject to phosphorylation by at least three protein kinases [Shi, H., Asher, C., Chigaev, A., Yung, Y., Reuveny, E., Seger, R. & Garty, H. (2002)J. Biol. , 13539–13547]. One of them was identified as ERK which phosphorylates bT613 andcT623 and affects the channel interaction with Nedd4 | Eur. J. Biochem. 269 4551-4558 2002 FEBS 2002 doi Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits Haikun Shi1 Carol Asher1 Yuval Yung2 Luba Kligman1 Eitan Reuveny1 Rony Seger2 and Haim Garty1 1 Department of Biological Chemistry and 2Department of Biological Regulation The Weizmann Institute of Science Rehovot Israel A number of findings have suggested the involvement of protein phosphorylation in the regulation of the epithelial Na channel ENaC . A recent study has demonstrated that the C tails of the b and y subunits of ENaC are subject to phosphorylation by at least three protein kinases Shi H. Asher C. Chigaev A. Yung Y. Reuveny E. Seger R. Garty H. 2002 J. Biol. Chem. 277 13539-13547 . One of them was identified as ERK which phosphorylates bT613 and yT623 and affects the channel interaction with Nedd4. The current study identifies a second protein kinase as casein kinase 2 CK2 or CK-2-like kinase. It phosphorylates bS631 a well-conserved serine on the b subunit. Such phosphorylation is observed both in vitro using glutathi-one-S-transferase-ENaC fusion proteins and in vivo in ENaC-expressing Xenopus oocytes. The y subunit is weakly phosphorylated by this protein kinase on another residue cT599 and the C tail of a is not significantly phosphorylated by this kinase. Thus CK2 may be involved in the regulation of the epithelial Na channel. Keywords casein kinase 2 ENaC epithelial Na channel phosphorylation. Active Na reabsorption in kidney collecting duct distal colon lung and exocrine glands is mediated by an apical Na specific channel termed ENaC epithelial Na -channel 1-3 . The channel is a major target of the action of several hormones such as the mineralocorticoid aldosterone the anti-diuretic peptide vasopressin and insulin 1 4 . It is composed of three homologous subunits a b and y which transverse the membrane twice so that both the C and N termini are intracellular 5-8 . Cell .

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