TAILIEUCHUNG - Báo cáo khoa học: b-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH

Amyloid protein (Ab1–40) aggregation and conformation was examined using native and sodium dodecyl sulfate/ polyacrylamide gel electrophoresis,and the results com-pared with those obtained by atomic force microscopy, and with Congo red binding,sedimentation and turbidity assays. The amount of Ab aggregation measured was different,depending upon the method used. Incubation for 15 min at pH or in the presence of Fe 2+ ,Cu 2+ or Zn 2+ did not alter the level of Aboligomers observed on SDS and native gels | Eur. J. Biochem. 270 228 2-223 2003 FEBS 2003 doi p-Amyloid protein oligomers induced by metal ions and acid pH are distinct from those generated by slow spontaneous ageing at neutral pH Genevieve M. J. A. Klug1 Dusan Losic2 3 Supundi S. Subasinghe1 2 Marie-Isabel Aguilar2 Lisandra L. Martin3 and David H. Small2 1 Department of Pathology University of Melbourne Victoria Australia 2Department of Biochemistry and Molecular Biology Monash University Victoria Australia 3Department of Chemistry Flinders University Adelaide Australia Amyloid protein Ab1-40 aggregation and conformation was examined using native and sodium dodecyl sulfate polyacrylamide gel electrophoresis add the eesults compared with those obtained by atomic force microscopy and with Congo red binding hedimantaiion and Surbidity assays. The amount of Ab aggregation measured was different de haedirg Lioon die me od seed. 1 1 111011 for 15 min at pH or in the presence of Fe2 Cu2 or Zn2 did not alter the level of Ab oligomers observed on SDS and native gels. However die slow aggregatim of Ab to form high molecular mass species over 5 days was inhibited. In contrast wIiou ab aggregation was monitored using a Congo red binding assay or sedimentation assay a rapid increase in A b aggregation was observed after incubation for 15 min at pH or in the pe onee of Fe2 uu2 or Zn2 . The low pH- Zn2 - or Cu2 -induced Ab aggregation measured in a turbidity assay was reversible. In contrast a considerable proportion of the Ab aggregation measured by native and SDS PAGE was stable. Atomic force microscopy studies showed that Ab aged at pH or in the presence of Zn2 produced larger looser rod-shaped aggregates than at pH . Ab that had been aged at pH was more cytotoxic than Ab aged at pH . Taken together tìie esllits u e OKit Ab oligomerizes via two mutually exclusive mechanisms to form two different types of aggregates which differ in their cytotoxic properties. .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• medicine
• cell proliferation
• oxidation
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes