TAILIEUCHUNG - The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged cultures

This document has 6 content: introduction, materials and methods, results and discussion, conclusion,acknowledgements, references. | Process Biochemistry Vol. 33. No. 5. pp. 547-553. 1998 1998 Elsevier Science Ltd. All rights reserved Printed in Great Britain 0032-9592 98 ELSEVIER p I I 80032-9592 98 00023-5 The influence of environmental conditions on polysaccharide formation by Ganoderma lucidum in submerged cultures Fan-Chiang Yang and Chun-Bun Liau Department of Chemical Engineering Tunghai University Taichung Taiwan 40704 Received 16 September 1997 revised version received 13 January 1998 accepted 31 January 1998 Abstract The effects of environmental parameters on polysaccharide formation by Ganoderma lucidunt were investigated in submerged cultures. The optimal temperature and pH was 30-35 C and 4-4 5 respectively in a glucose-ammonium chloride medium and polysaccharide concentration reached l 6mg ml. Agitation and aeration influenced the formation and secretion of polysaccharide. The optimal rotating speed was 150 rpm in 7-day flask cultures while the agitation speed employed in fermenter culture greatly affected the production rate and maximum concentration of polysaccharide. Although higher speeds enhanced mixing efficiency and polysaccharide release higher shear stress had a detrimental effect on mycelial growth and polysaccharide formation. 1998 Elsevier Science Ltd. All rights reserved Keywords Ganoderma lucidum polysaccharide submerged culture. Introduction Ganoderma lucidum Fr. Krast Polyporaceae and related species are fungi used in traditional Chinese medicine. Its fruiting body is called Reishi or Man-nentakc in Japanese and Lingzhi in China. In regions of China Japan Korea and Taiwan Lingzhi has been a popular folk or oriental medicine used to treat various human diseases such as hepatitis hypertension hypercholesterolemia and gastric cancer. Recent studies on this fungus have demonstrated many interesting biological activities including antitumour and anti-inflammatory effects and cytotoxicity to hepatoma cells. These studies also suggested that the .

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