TAILIEUCHUNG - Introduction to Modern Liquid Chromatography, Third Edition part 6

Introduction to Modern Liquid Chromatography, Third Edition part 6. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 6 INTRODUCTION Figure Recent examples of HPLC capability a Fast separation of six proteins using gradient elution with a 150 x column packed with im-diameter pellicular particles 5 b initial separation of peptides and proteins from human fetal fibroblast cell by gradient cation-exchange chromatography c further separation of fraction 7 collected between 24-28 min on a second column by gradient reversed-phase chromatography 6 . Figures adapted from original publications 5 6 . field of application andfacilitatedmajor advancesinbiochenustty. Simiiarlythe development ofclnral columnc ioitlicscparatioiioCciiaiifiomeoitmixiurcs byPtrktc 9 and othcrscaabled compacahlcarleanacc in time arcasofphatmaceciticals and related lifecciences he usaof HPLCfor large-scale purification is also increasing as a result of the availability of appropriate equipment an increase in our understanding of how suchsepafetionf 111 0 1 1 bc cocried eat cndregulatorycteisuaas foe higher puritephermrceuticcl crrtucti. A SHORT HISTORYOF HPLC We have noted tlie developmentof liqcMchromatography prior to the advent of HPLC Section . For a more complete account of this pre-1965 period several review articles have been written by Leslie Ettre our historian of chromatography precisors to t hrom ogr h deveb entsprior to 1900 10 11 invention of chronuto aphy by M. T TswCtt m the early IWOs fl2 A SHORT HISTORY OF HPLC 7 rediscovery of chromatography in the early 1930s 13 A. J. P. Martin s invention of partition and paper chromatography in the early 1940s 14 development of the amino-acid analyzer by S. Moore and W. S. Stein in the late 1950s 15 development ofthe gel-permeationchromatograph byWatersAssociates in the early 1960s 16 Carl Runye e in tS5i Sbsr repttterdcr separations by means of a technique similar to paper chromatography 10 but neither he nor others pursurdthe practical possibilities of this work. In the late 1890s David Day trhhe Ua Gnologirel

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