TAILIEUCHUNG - Báo cáo khoa học: Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation

Selenium is a trace element found in freshwater and the marine environ-ment. We show that it plays a major role in spicule formation in the demo-sponge Suberites domuncula. If added to primmorphs, an in vitro sponge cell culture system, it stimulates the formation of siliceous spicules. Using differential display of transcripts, we demonstrate that, after a 72-h expo-sure of primmorphs to selenium, two genes are up-regulated; one codes for selenoprotein M and the other for a novel spicule-associated protein | iFEBS Journal Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation Werner E. G. Muller1 Alexandra Borejko1 David Brandt1 Ronald Osinga2 Hiroshi Ushijima3 Bojan Hamer4 Anatoli Krasko1 Cao Xupeng1 Isabel M. Muller1 and Heinz C. Schroder1 1 Institut fur Physiologische Chemie Abteilung Angewandte Molekularbiologie Universitat Mainz Germany 2 Wageningen University Fish Culture and Fisheries Group the Netherlands 3 Department of DevelopmentalMedicalSciences Institute of InternationalHealth Graduate Schoolof Medicine University of Tokyo Japan 4 Center for Marine Research Ruder Boskovic Institute Rovinj Croatia Keywords selenium silica silicatein spicules sponges Correspondence W. E. G. Muller Institut fur Physiologische Chemie Abteilung Angewandte Molekularbiologie Universitat Duesbergweg 6 55099 Mainz Germany Fax 49 6131 3925243 Tel 49 6131 3925910 E-mail wmueller@ Received 24 March 2005 revised 18 May 2005 accepted 26 May 2005 Note The cDNA sequences for selenoprotein M AJ875186 and spicule-associated protein AJ872182 have been deposited at EMBL GenBank. doi Selenium is a trace element found in freshwater and the marine environment. We show that it plays a major role in spicule formation in the demosponge Suberites domuncula. If added to primmorphs an in vitro sponge cell culture system it stimulates the formation of siliceous spicules. Using differential display of transcripts we demonstrate that after a 72-h exposure of primmorphs to selenium two genes are up-regulated one codes for selenoprotein M and the other for a novel spicule-associated protein. The deduced protein sequence of selenoprotein M 14 kDa shows characteristic features of metazoan selenoproteins. The spicule-associated protein 26 kDa comprises six characteristic repeats of 20 amino acids composed of 10 distinct hydrophobic regions w 9 amino acids in length . Recombinant proteins were .

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