TAILIEUCHUNG - Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase

Taurocyamine kinase (TK) is a member of the highly conserved family of phosphagen kinases that includes creatine kinase (CK) and arginine kinase. TK is found only in certain marine annelids. In this study we used PCR to amplify two cDNAs coding for TKs from the polychaeteArenicola brasil-iensis, cloned these cDNAs into the pMAL plasmid and expressed the TKs as fusion proteins with the maltose-binding protein. | ềFEBS Journal Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase Kouji Uda1 Naoto Saishoji1 Shuichi Ichinari1 W. Ross Ellington2 and Tomohiko Suzuki1 1 Laboratory of Biochemistry Faculty of Science Kochi University Japan 2 Institute of Molecular Biophysics and Department of BiologicalScience Florida State University Tallahassee FL USA Keywords taurocyamine kinase creatine kinase phosphagen kinase cDNA sequence mitochondrial Correspondence T. Suzuki Laboratory of Biochemistry Faculty of Science Kochi University Kochi 780-8520 Japan Fax 81 88 844 8356 Tel 81 88 844 8693 E-mail suzuki@ Received 9 March 2005 revised 23 April 2005 accepted 13 May 2005 doi Taurocyamine kinase TK is a member of the highly conserved family of phosphagen kinases that includes creatine kinase CK and arginine kinase. TK is found only in certain marine annelids. In this study we used PCR to amplify two cDNAs coding for TKs from the polychaete Arenicola brasil-iensis cloned these cDNAs into the pMAL plasmid and expressed the TKs as fusion proteins with the maltose-binding protein. These are the first TK cDNA and deduced amino acid sequences to be reported. One of the two cDNA-derived amino acid sequences of TKs shows a high amino acid identity to lombricine kinase another phosphagen kinase unique to annelids and appears to be a cytoplasmic isoform. The other sequence appears to be a mitochondrial isoform it has a long N-terminal extension that was judged to be a mitochondrial targeting peptide by several on-line programs and shows a higher similarity in amino acid sequence to mitochondrial creatine kinases from both vertebrates and invertebrates. The recombinant cytoplasmic TK showed activity for the substrates taurocyamine and lombricine 9 of that of taurocyamine . However the mitochondrial TK showed activity for taurocyamine lombricine 30 of that of taurocyam-ine and glycocyamine 7 of that of taurocyamine . .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• immune system
• Antimicrobial
• biosynthesis
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes