TAILIEUCHUNG - Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide.

Photoactivated riboflavin in the presence of Cu(II) generates reactive oxygen species (ROS) which can hemolyse human red blood cells (RBC). In the present work we examined the effect of sodium azide (NaN3) on RBC in the presence of riboflavin and Cu(II). The addition of NaN3 to the riboflavin-Cu(II) sys-tem enhanced K + loss and hemolysis. The extent of K + loss and hemolysis were time and concentration dependent. Batho-cuproine, a Cu(I)-sequestering agent inhibited the hemolysis completely. | Abstracts V1-Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu II system enhancement by azide. I. Ali1 and I. Naseem2 IFaculty of Pharmacy Applied Science university Amman Amman Jordan 2Department of Biochemistry Faculty of Life Sciences Aligarh Muslim University Aligarh UP India. E-mail iyad74@ Photoactivated riboflavin in the presence of Cu II generates reactive oxygen species ROS which can hemolyse human red blood cells RBC . In the present work we examined the effect of sodium azide NaN3 on RBC in the presence of riboflavin and Cu II . The addition of NaN3 to the riboflavin-Cu II system enhanced K loss and hemolysis. The extent of K loss and hemolysis were time and concentration dependent. Batho-cuproine a Cu I -sequestering agent inhibited the hemolysis completely. Among various free radical scavengers used to identify the major ROS involved in the reaction thiourea was found to be the most effective scavenger. Thiourea caused almost 85 inhibition of hemolysis suggesting that OH is the major ROS involved in the reaction. Using spectral studies and other observations we propose that when NaN3 is added to riboflavin-Cu II system it inhibits the photodegradation of riboflavin resulting in increased OH generation. Also the possibility of azide radical formation and its involvement in the reaction could not be ruled out. V1-002P Involvement of thromboxane A2 synthase in the dexamethasone altered response to electrical field stimulation in mesenteric arteries from spontaneously hypertensive rats R. M. Aras M. Ferrer and G. Balfagon Departamento de Fisiologia Universidad Autonoma de Madrid Madrid Spain. E-mail arasmadrid@ The aim of this study was to analyze whether dexamethasone alters the vasoconstrictor response induced by electrical field stimulation EFS in endothelium denuded mesenteric arteries from spontaneously hypertensive rats SHR . For this purpose superior mesenteric arteries from male SHR rats were used to analyze .

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