TAILIEUCHUNG - Báo cáo khoa học: Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes

Transmembrane proton translocation in the photosynthetic membranes of the purple bacteriumRhodobacter sphaero-ides is driven by light and performed by two transmem-brane complexes; the photosynthetic reaction center and the ubiquinol–cytochromecoxidoreductase complex, coupled by two mobile electron carriers; the cytochrome and the quinone. This paper focuses on the kinetics and thermo-dynamics of the interaction between the lipophylic electron carrier ubiquinone-10 and the photosynthetic enzyme reconstituted in liposomes. . | Eur. J. Biochem. 270 4595-4605 2003 FEBS 2003 doi Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes Francesco Milano1 Angela Agostiano1 2 Fabio Mavelli2 and Massimo Trotta1 1CNR Istituto per i Processi Chimico-Fisici - Sezione di Bari and 2Dipartimento di Chimica Universitá di Bari Italy Transmembrane proton translocation in the photosynthetic membranes of the purple bacterium Rhodobacter sphaero-ides is driven by light and performed by two transmembrane complexes the photosynthetic reaction center and the ubiquinol-cytochrome c oxidoreductase complex coupled by two mobile electron carriers the cytochrome and the quinone. This paper focuses on the kinetics and thermodynamics of the interaction between the lipophylic electron carrier ubiquinone-10 and the photosynthetic enzyme reconstituted in liposomes. The collected data were simulated with an existing recognized kinetic scheme Shinkarev . Wraight . 1993 In The Photosynthetic Reaction Center Deisenhofer J. Norris . eds. pp. 193- 255. Academic Press San Diego CA USA and the kinetic constants of the uptake X 107 M-1-s-1 and release 40 s 1 processes of the ligand were inferred. The results obtained for the quinone release kinetic constant are comparable to the rate of the charge recombination reaction from the state D Qa-. Values for the kinetic constants are discussed as part of the overall photocycle suggesting that its bottleneckmay not be the quinone uptaek reaction in agreement with a previous report Gerencser L. Laczko G. Maroti P. 1999 Biochemistry 38 16866-16875 . Keywords reaction center quinone binding liposomes photosynthesis. The photosynthetic apparatus of the nonsulfur purple bacterium Rhodobacter sphaeroides sits primarily in dedicated portions of the cell membrane called intracytoplasmatic membranes ICM 1 2 . The key enzymes involved in the build-up of the

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