TAILIEUCHUNG - Báo cáo khoa học: Selection strategies for improved biocatalysts

Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However, their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. | ỊFEBS Journal REVIEW ARTICLE Selection strategies for improved biocatalysts Ykelien L. Boersma1 Melloney J. Droge2 and Wim J. Quax1 1 Department of PharmaceuticalBiology Groningen University Institute for Drug Exploration GUIDE the Netherlands 2 ABL BV Assen the Netherlands Keywords cell surface display directed evolution enzymes genetic selection in vitro compartmentalization phage display ribosome display Correspondence W. J. Quax Department of Pharmaceutical Biology Groningen University Institute for Drug Exploration GUIDE University of Groningen A. Deusinglaan 1 9713 AV Groningen the Netherlands Fax 31 50 3633000 Tel 31 50 3632558 E-mail Received 31 January 2007 accepted 9 March 2007 doi Enzymes have become an attractive alternative to conventional catalysts in numerous industrial processes. However their properties do not always meet the criteria of the application of interest. Directed evolution is a powerful tool for adapting the characteristics of an enzyme. However selection of the evolved variants is a critical step and therefore new strategies to enable selection of the desired enzymatic activity have been developed. This review focuses on these novel strategies for selecting enzymes from large libraries in particular those that are used in the synthesis of pharmaceutical intermediates and pharmaceuticals. Introduction Directed evolution Over the past few decades it has become clear that enzymes hold great potential for industry. They are among the most remarkable biomolecules known because of their extraordinary specificity and catalytic power 1 . The specificity and enantio- and regio- selectivity of certain enzymatic transformations makes them attractive for the production of fine chemicals and pharmaceutical intermediates. To date more than 500 products over a wide spectrum of applications are manufactured by enzymes. Well-known examples are ephedrine aspartame and amoxicillin 2 3 . However in many .

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