TAILIEUCHUNG - Báo cáo khoa học: Sulfatide with short fatty acid dominates in astrocytes and neurons

Glycosphingolipids are located in cell membranes and the brain is especi-ally enriched. We speculated that the subcellular location of glycosphingo-lipids depends on their fatty acid chain length because their sugar residues are constant, whereas fatty acid chain length can vary within the same molecule. | iFEBS Journal Sulfatide with short fatty acid dominates in astrocytes and neurons Giorgis Isaac1 Zarah Pernber2 Volkmar Gieselmann3 Elisabeth Hansson2 Jonas Bergquist1 and Jan-Eric Mansson2 1 Department of AnalyticalChemistry Uppsala University Sweden 2 Institute of ClinicalNeuroscience Sahlgrenska University Hospital Goteborg University Molndal Sweden 3 Institut fur Physiologische Chemie Universitat Bonn Germany Keywords metachromatic leukodystrophy neuron astrocyte stearic acid vesicle Correspondence J. Bergquist Department of Analytical Chemistry Uppsala University PO Box 599 SE-751 24 Uppsala Sweden Fax 46 18 471 3692 Tel 46 18 471 3695 E-mail Present address Division of Biology Kansas State University Manhattan 66506 USA Received 22 December 2005 revised 17 February 2006 accepted 22 February 2006 doi Glycosphingolipids are located in cell membranes and the brain is especially enriched. We speculated that the subcellular location of glycosphingolipids depends on their fatty acid chain length because their sugar residues are constant whereas fatty acid chain length can vary within the same molecule. To test this hypothesis we analysed the glycosphingolipid sulfatide which is highly abundant in myelin and has mostly long fatty acids. We used a negative ion electrospray tandem mass spectrometry precursor ion scan to analyse the molecular species of sulfatide in cultured astrocytes and a mouse model of the human disease metachromatic leukodystrophy. In these arylsulfatase A ASA -deficient mice sulfatide accumulates intracellularly in neurons and astrocytes. Immunocytochemistry was also performed on cultured astrocytes and analysed using confocal laser scanning microscopy. Analyses of the molecular species showed that cultured astrocytes contained sulfatide with a predominance of stearic acid C18 which was located in large intracellular vesicles throughout the cell body and along the processes. The same was

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