TAILIEUCHUNG - Báo cáo khoa học: Roles of base excision repair subpathways in correcting oxidized abasic sites in DNA

Base excision DNA repair (BER) is fundamentally important in handling diverse lesions produced as a result of the intrinsic instability of DNA or by various endogenous and exogenous reactive species. Defects in the BER process have been associated with cancer susceptibility and neurodegenera-tive disorders. | iFEBS Journal MINIREVIEW Roles of base excision repair subpathways in correcting oxidized abasic sites in DNA Jung-Suk Sung1 and Bruce Demple2 1 Department of Life Science Dongguk University Seoul South Korea 2 Department of Genetics and Complex Diseases Harvard Schoolof Public Health Boston MA USA Keywords 2-deoxyribonolactone DNA polymerase beta DNA-protein crosslinks FEN1 protein long-patch BER oxidized abasic sites short-patch BER Correspondence B. Demple Department of Genetics and Complex Diseases Harvard Schoolof Public Health Boston MA 02115 USA Fax 1 617 432 0377 Tel 1 617 432 3462 E-mail bdemple@ Received 12 December 2005 accepted 6 February 2006 doi Base excision DNA repair BER is fundamentally important in handling diverse lesions produced as a result of the intrinsic instability of DNA or by various endogenous and exogenous reactive species. Defects in the BER process have been associated with cancer susceptibility and neurodegenera-tive disorders. BER funnels diverse base lesions into a common intermediate apurinic apyrimidinic AP sites. The repair of AP sites is initiated by the major human AP endonuclease Ape1 or by AP lyase activities associated with some DNA glycosylases. Subsequent steps follow either of two distinct BER subpathways distinguished by repair DNA synthesis of either a single nucleotide short-patch BER or multiple nucleotides long-patch BER . As the major repair mode for regular AP sites the short-patch BER pathway removes the incised AP lesion a 5 -deoxyribose-5-phosphate moiety and replaces a single nucleotide using DNA polymerase Polp . However short-patch BER may have difficulty handling some types of lesions as shown for the C1 -oxidized abasic residue 2-deoxyribonolactone dL . Recent work indicates that dL is processed efficiently by Ape1 but that short-patch BER is derailed by the formation of stable covalent crosslinks between Ape1-incised dL and Polp. The long-patch BER .

TÀI LIỆU LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.