TAILIEUCHUNG - Báo cáo khoa học: Submembraneous microtubule cytoskeleton: regulation of ATPases by interaction with acetylated tubulin

The ATP-hydrolysing enzymes (Na + ,K + )-, H + - and Ca 2+ -ATPase are integral membrane proteins that play important roles in the exchange of ions and nutrients between the exterior and interior of cells, and are involved in signal transduction pathways. Activity of these ATPases is reg-ulated by several specific effectors. | ễFEBS Journal MINIREVIEW Submembraneous microtubule cytoskeleton regulation of ATPases by interaction with acetylated tubulin Carlos A. Arce1 Cesar H. Casale2 and Hector S. Barra1 1 Centro de Investigaciones en Quimica Biologica de Cordoba Departamento de Quimica Biologica Universidad Nacionalde Cordoba Argentina 2 Departamento de Biologia Molecular Facultad de Ciencias Exactas Fisico-Quimicas y Naturales Universidad Nacionalde Rio Cuarto Coi rdoba Argentina Keywords acetylated tubulin C Ca2 -ATPase cytoskeleton H -ATPase membranous tubulin microtubules P-type ATPases signal transduction tubulin Correspondence H. S. Barra Departamento de Quimica Biologica Facultad de Ciencias Quimicas Universidad Nacionalde Cordoba Ciudad Universitaria 5000-Cordoba Argentina Fax 54 351 433 4168 4074 Tel 54 351 433 4168 4074 E-mail hbarra@ Received 15 April2008 revised 5 June 2008 accepted 30 July 2008 The ATP-hydrolysing enzymes Na K - H - and Ca2 -ATPase are integral membrane proteins that play important roles in the exchange of ions and nutrients between the exterior and interior of cells and are involved in signal transduction pathways. Activity of these ATPases is regulated by several specific effectors. Here we review the regulation of these P-type ATPases by a common effector acetylated tubulin which interacts with them and inhibits their enzyme activity. The presence of an acetyl group on Lys40 of a-tubulin is a requirement for the interaction. Stimulation of enzyme activity by different effectors involves the dissociation of tubulin ATPase complexes. In cultured cells acetylated tubulin associated with ATPase appears to be a constituent of microtubules. Stabilization of microtubules by taxol blocks association dissociation of the complex. Membrane ATPases may function as anchorage sites for microtubules. doi Among the huge number of membrane ATPases those of the P-type so termed because they undergo autophosphorylation .

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