TAILIEUCHUNG - Báo cáo khoa học: Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin

Lectins belonging to the jacalin-related lectin family are distributed widely in the plant kingdom. Recently, two mannose-specific lectins having tan-dem repeat-type structures were discovered in Castanea crenata(angio-sperm) and Cycas revoluta(gymnosperm). The occurrence of such similar molecules in taxonomically less related plants suggests their importance in the plant body. | iFEBS Journal Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins Castanea crenata agglutinin and Cycas revoluta leaf lectin Sachiko Nakamura1 Fumio Yagi2 Kiichiro Totani3 Yukishige Ito3 and Jun Hirabayashi1 1 Glycostructure Analysis Team Research Center for Glycoscience National institute of Advanced Industrial science and Technology Japan 2 Department of BiochemicalScience and Technology Faculty of Agriculture Kagoshima University Japan 3 RIKEN The Institute of Physicaland ChemicalResearch Saitama Japan Keywords carbohydrate binding specificity frontal affinity chromatography Jacalin-related lectin family lectin Correspondence J. Hirabayashi Glycostructure Analysis Team Research Center for Glycoscience National institute of Advanced Industrial Science and Technology AIST Tsukuba Central2 1-1-1 Umezono Tsukuba Ibaraki 305-8568 Japan Fax 81 29 861 3125 Tel 81 29 861 3124 E-mail jun-hirabayashi@ Received 27 December 2004 revised 23 February 2005 accepted 4 April 2005 doi Lectins belonging to the jacalin-related lectin family are distributed widely in the plant kingdom. Recently two mannose-specific lectins having tandem repeat-type structures were discovered in Castanea crenata angiosperm and Cycas revoluta gymnosperm . The occurrence of such similar molecules in taxonomically less related plants suggests their importance in the plant body. To obtain clues to understand their physiological roles we performed detailed analysis of their sugar-binding specificity. For this purpose we compared the dissociation constants Kd of Castanea crenata agglutinin CCA and Cycas revoluta leaf lectin CRLL by using 102 pyridylaminated and 13 p-nitrophenyl oligosaccharides with a recently developed automated system for frontal affinity chromatography. As a result we found that the basic carbohydrate-binding properties of CCA and CRLL were similar but differed in their preference for larger

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