TAILIEUCHUNG - Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes

Hepatocytes located in the periportal and perivenous zones of the liver lobule show remarkable differences in the levels and activities of various enzymes and other proteins. To analyze global gene expression patterns of periportal and perivenous hepatocytes, enriched populations of the two cell types were isolated by combined collagenase⁄digitonin perfusion from mouse liver and used for microarray analysis. | ỊFEBS Journal Differential gene expression in periportal and perivenous mouse hepatocytes Albert Braeuning1 Carina Ittrich2 Christoph Kohle1 Stephan Hailfinger1 Michael Bonin3 Albrecht Buchmann1 and Michael Schwarz1 1 Institute of Pharmacology and Toxicology Department of Toxicology University of Tuebingen Germany 2 CentralUnit of Biostatistics German Cancer Research Center Heidelberg Germany 3 Institute for Human Genetics Microarray Facility Tuebingen Germany Keywords metabolic zonation microarray analysis mouse liver zonalgene expression Correspondence M. Schwarz Institute of Pharmacology and Toxicology Department of Toxicology University of Tuebingen Wilhelmstr. 56 72074 Tuebingen Germany Fax 49 7071 29 2273 Tel 49 7071 29 77398 E-mail Received 24 July 2006 revised 15 September 2006 accepted 18 September 2006 doi Hepatocytes located in the periportal and perivenous zones of the liver lobule show remarkable differences in the levels and activities of various enzymes and other proteins. To analyze global gene expression patterns of periportal and perivenous hepatocytes enriched populations of the two cell types were isolated by combined collagenase digitonin perfusion from mouse liver and used for microarray analysis. In total 198 genes and expressed sequences were identified that demonstrated a 2-fold difference in expression between hepatocytes from the two different zones of the liver. A subset of 20 genes was additionally analyzed by real-time RT-PCR validating the results obtained by the microarray analysis. Several of the differentially expressed genes encoded key enzymes of intermediary metabolism including those involved in glycolysis and gluconeogenesis fatty acid degradation cholesterol and bile acid metabolism amino acid degradation and ammonia utilization. In addition several enzymes of phase I and phase II of xenobiotic metabolism were differentially expressed in periportal and .

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