TAILIEUCHUNG - Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression

Sponges (phylum Porifera) live in a symbiotic relationship with microorganisms, primarily bacteria. Until now, mole-cular proof for the capacity of sponges to recognize fungi in the surroundingaqueousmilieuhas not we demonstrate, for the demospongeSuberites domuncula (Porifera, Demospongiae, Hadromerida), a cell surface receptor that recognizes (1fi3)-b-D-glucans, . curdlan or laminarin. This receptor, the (1fi3)-b-D-glucan-binding protein, was identified and its cDNA analysed. The gene coding for the 45 kDa protein was found to be upregulated in tissue after incubationwith carbohydrate. . | Eur. J. Biochem. 271 1924-1937 2004 FEBS 2004 doi A 1fi3 -p-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression Sanja Perovic-Ottstadt1. Teresa Adell1 Peter Proksch2 Matthias Wiens1 Michael Korzhev1 Vera Gamulin3 Isabel M. Muller1 and Werner E. G. Muller1 1Institut fur Physiologische Chemie Abteilung Angewandte Molekularbiologie Universităt Mainz Germany 2Institut fur Pharmazeutische Biologie Heinrich-Heine-Universităt Dusseldorf Germany 3Institute Rudjer Boskovic Department of Molecular Biology Zagreb Croatia Sponges phylum Porifera live in a symbiotic relationship with microorganisms primarily bacteria. Until now molecular proof for the capacity of sponges to recognize fungi in the surrounding aqueous milieu has not been available. Here we demonstrate for the demosponge Suberites domuncula Porifera Demospongiae Hadromerida a cell surface receptor that recognizes 1fi3 -b-D-glucans . curdlan or laminarin. This receptor the 1fi3 -b-D-glucan-binding protein was identified and its cDNA analysed. The gene coding for the 45 kDa protein was found to be upregulated in tissue after incubation with carbohydrate. Simultaneously with the increased expression of this gene two further genes showed an elevated steady state level of expression one codes for a fibrinogen-like protein and the other for the epidermal growth factor precursor. Expression of the 1fi3 -b-D-glucan-binding protein and the fibrinogen-like protein occurred in cells on the sponge surface in the pin-acoderm. By Western blotting the product of the fibrinogen-like protein gene was identified the recombinant protein isolated and antibodies raised to this protein. Their application revealed that a 5 kDa factor is produced which is apparently processed from the 77 kDa epidermal growth factor precursor. Finally we provided evidence that a tyrosine kinase pathway is initiated in

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