TAILIEUCHUNG - Báo cáo khoa học: Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss)

A homologue of interleukin 18 has been identified from rainbow trout,Oncorhynchus mykiss. The trout IL-18gene spans kb and consists of six exons and five introns, sharing the same gene organization with its human coun-terpart. The putative translated protein is 199 amino acids in length with no predicted signal peptide. Analysis of the multiple sequence alignment reveals a conserved ICE cut site, resulting in a mature peptide of 162 amino acids. The trout IL-18 shares 41–45% similarity with known IL-18 molecules and contains an IL-1 family signature motif | Eur. J. Biochem. 271 1913-1923 2004 FEBS 2004 doi Identification and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout Oncorhynchus mykiss Jun Zou1. Steve Bird1 Jonathan Truckle1 Niels Bols2 Mike Horne3 and Chris Secombes1 1 Scottish Fish Immunology Research Centre School of Biological Sciences University of Aberdeen UK department of Biology University of Waterloo Ontario Canada 3Novartis Aquahealth Enterprise House Springkerse Business Park Stirling UK A homologue of interleukin 18 has been identified from rainbow trout Oncorhynchus mykiss. The trout IL-18 gene spans kb and consists of six exons and five introns sharing the same gene organization with its human counterpart. The putative translated protein is 199 amino acids in length with no predicted signal peptide. Analysis of the multiple sequence alignment reveals a conserved ICE cut site resulting in a mature peptide of 162 amino acids. The trout IL-18 shares 41-45 similarity with known IL-18 molecules and contains an IL-1 family signature motif. It is constitutively expressed in a wide range of tissues including brain gill gut heart kidney liver muscle skin and spleen. Transcription is not modulated by lipopolysaccharide poly I C or trout recombinant IL-1b in primary head kidney leucocyte cultures and RTS-11 cells a macrophage cell line. However expression is downregulated by lipopolysaccharide and rIL-1 b in RTG-2 cells a fibroblast-like cell line. An alternatively spliced form of IL-18 mRNA has also been found and translates into a 182 amino acid protein with a 17 amino acid deletion in the precursor region of the authentic form. This alternatively spliced form is also widely expressed although much lower than the authentic form. Interestingly its expression is upregulated by lipopolysaccharide and poly I C but is not affected by rIL-1b in RTG-2 cells. The present study suggests that alternative splicing may play an important

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