TAILIEUCHUNG - Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells

Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate frac-tions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associ-ated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. . | iFEBS Journal Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells Martin R11 m hn1 f Fra nrnico r m I ria 11 lt-fi ai itĩor2 PráHórir .Qíorrn1 loan-Clai ido RirarH1 S IVI a I IIl I 11 IUI 11 u o I Ian Cr oi z x-z VZ J I j a Jii j a Jii z I I e xJ e I I z I e I I oz I eai Ixziauuu o I I a I xJ z and Emanuela Felley-Bosco2 1 Swiss ExperimentalCancer Research Center Epalinges Switzerland 2 Institute of Pharmacology and Toxicology Lausanne Switzerland Keywords dimerization inducible nitric oxide synthase intestinalepithelialcells specific activity subcellular distribution Correspondence E. Felley-Bosco Institute of Pharmacology and Toxicology Rue du Bugnon 27 1005 Lausanne Switzerland Fax 41 21 6925355 Tel 41 21 6925370 E-mail These authors contributed equally to the work described. Present addresses tDepartamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacionalde La Plata Argentina tThe Garvan Institute of MedicalResearch Darlinghurst Australia Institut de Biologie de Lille Groupe AVENIR Equipe Mixte INSERM Universite E0364 Lille France Received 16 September 2004 revised 15 November 2004 accepted 16 November 2004 doi Inducible nitric oxide synthase iNOS functions as a homodimer. In cell extracts iNOS molecules partition both in cytosolic and particulate fractions indicating that iNOS exists as soluble and membrane associated forms. In this study iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. In particulate fractions iNOS dimers were more abundant than in the cytosolic fraction. Similar observations were seen in mouse .

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