TAILIEUCHUNG - Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life

The methanogenic archaeaMethanococcus jannaschiiand M. maripaludiscontain an atypical seryl-tRNA synthetase (SerRS), which recognizes eukaryotic and bacterial tRNAs Ser , in addition to the homologous tRNA Ser and tRNA Sec relativeflexibility in tRNArecognition displayed by methanogenic SerRSs, shown by aminoacyla-tion and gel mobility shift assays, indicates the conservation of some serine determinants in all three domains. The complex of M. maripaludisSerRS with the homologues tRNA Ser was isolated by gel filtration chromatography | Eur. J. Biochem. 271 694-702 2004 FEBS 2004 doi The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life Silvija Bilokapic1 2 Dragana Korencic2 3 Dieter Soil3 and Ivana Weygand-Durasevic1 2 1 Department of Chemistry Faculty of Science University of Zagreb Croatia 2Rudjer Boskovic Institute Zagreb Croatia 3Department of Molecular Biophysics and Biochemistry Yale University New Haven CT USA The methanogenic archaea Methanococcus jannaschii and M. maripaludis contain an atypical seryl-tRNA synthetase SerRS which recognizes eukaryotic and bacterial tRNAsSer in addition to the homologous tRNASer and tRNASec species. The relative flexibility in tRNA recognition displayed by methanogenic SerRSs shown by aminoacylation and gel mobility shift assays indicates the conservation of some serine determinants in all three domains. The complex of M. maripaludis SerRS with the homologues tRNASer was isolated by gel filtration chromatography. Complex formation strongly depends on the conformation of tRNA. Therefore the renaturation conditions for in vitro transcribed tRNASerGCU isoacceptor were studied carefully. This tRNA unlike many other tRNAs is prone to dimerization possibly due to several stretches of complementary oligonucleotides within its sequence. Dimerization is facilitated by increased tRNA concentration and can be diminished by fast renaturation in the presence of 5 mM magnesium chloride. Keywords methanogenic archaea seryl-tRNA synthetase tRNA dimerization tRNASer recognition. Fidelity of translation depends on accurate charging by aminoacyl-tRNA synthetases. Investigations carried out in recent years on prokaryotic and eukaryotic aminoacylation systems have shown that the specificity of the aminoacylation reaction is correlated with the presence of a set of recognition elements which is largely conserved among species 1 . Great effort has been undertaken recently to unravel tRNA .

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