TAILIEUCHUNG - Safety Assessment of Roundup ReadyCorn Event NK603

Models are an initial useful starting point and can assist in a more structured and ordered approach to the planning of events. There is no one model which fits all. It is for the event organizer to perhaps select and engage with a model he / she finds useful, and one which they can understand, share with their colleagues / stakeholders and add to or detract from. These models are not set in stone, they need to be reviewed, but they may well assist as a reference point. As a visual representation of the key areas of the event planning. | Safety Assessment of Roundup Ready Corn Event NK603 Executive Summary Using modern biotechnology Monsanto Company has developed Roundup Ready corn plants that confer tolerance to glyphosate the active ingredient in Roundup agricultural herbicides by the production of the glyphosate-tolerant CP4 5-enolpyruvylshikimate-3-phosphate synthase EPSPS proteins. Glyphosate kills plants by inhibiting the enzyme EPSPS. This enzyme catalyzes a critical step in the shikimic acid pathway for the biosynthesis of aromatic amino acids in plants and microorganisms and its inhibition leads to the lack of growth in plants. The CP4 EPSPS proteins have a low affinity for glyphosate compared to the wild-type EPSPS enzyme. Thus when corn plants expressing the CP4 EPSPS proteins are treated with glyphosate the plants continue to grow. The continued action of the tolerant CP4 EPSPS enzyme provides the plant s need for aromatic acids. Aromatic amino acid biosynthesis is not present in animals. This explains the selective activity in plants and contributes to the low mammalian toxicity of glyphosate. Two copies of the cp4 epsps gene were introduced into the corn genome to produce Roundup Ready corn event NK603. The cp4 epsps gene derived from the common soil bacterium Agrobacterium sp. strain CP4 encodes for the naturally glyphosate-tolerant EPSPS protein. The food and feed safety of corn event NK603 was established based upon the evaluation of CP4 EPSPS activity and homology to EPSPS proteins present in a diversity of plants including those used for foods the low dietary exposure to CP4 EPSPS the rapid digestibility of CP4 EPSPS and the lack of toxicity or allergenicity of EPSPSs generally and by safety studies of the expressed CP4 EPSPS proteins. The equivalence of corn event NK603 compared to conventional corn was demonstrated by analyses of key nutrients including protein fat carbohydrates moisture amino acids fatty acids and minerals. Nutritional equivalence of corn event NK603 compared

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