TAILIEUCHUNG - Báo cáo khóa học: Some properties of human small heat shock protein Hsp20 (HspB6)

Human heat shock protein of apparent molecular mass 20 kDa (Hsp20) and its mutant, S16D, mimicking phos-phorylation by cyclic nucleotide-dependent protein kinases, were cloned and expressed inEscherichia coli. The proteins were obtained in a homogeneous state without utilization of urea or detergents. On size exclusion chromatography at neutral pH, Hsp20 and its S16D mutant were eluted as symmetrical peaks with an apparent molecular mass of 55–60 kDa. | Eur. J. Biochem. 271 291-302 2004 FEBS 2003 doi Some properties of human small heat shock protein Hsp20 HspB6 Olesya V. Bukach1 Alim S. Seit-Nebi1 Steven B. Marston2 and Nikolai B. Gusev1 1 Department of Biochemistry School of Biology Moscow State University Moscow Russia imperial College School of Medicine at National Heart and Lung Institute London UK Human heat shock protein of apparent molecular mass 20 kDa Hsp20 and its mutant S16D mimicking phosphorylation by cyclic nucleotide-dependent protein kinases were cloned and expressed in Escherichia coli. The proteins were obtained in a homogeneous state without utilization of urea or detergents. On size exclusion chromatography at neutral pH Hsp20 and its S16D mutant were eluted as symmetrical peaks with an apparent molecular mass of 55-60 kDa. Chemical crosslinking resulted in the formation of dimers with an apparent molecular mass of 42 kDa. At pH Hsp20 and its S16D mutant dissociated and were eluted in the form of two peaks with apparent molecular mass values of 45-50 and 28-30 kDa. At pH the chaperone activity of Hsp20 measured by its ability to prevent the reduction-induced aggregation of insulin or heat-induced aggregation of yeast alcohol dehydrogenase was similar to or higher than that of commercial a-crystallin. Under these conditions the S16D mutant of Hsp20 possessed lower chaperone activity than the wild-type protein. At pH both a-crystallin and Hsp20 interacted with denatured alcohol dehydrogenase however a-crystallin prevented whereas Hsp20 either did not affect or promoted the heat-induced aggregation of alcohol dehydrogenase. The mixing of wild-type human Hsp27 and Hsp20 resulted in a slow temperaturedependent formation of hetero-oligomeric complexes with apparent molecular mass values of 100 and 300 kDa which contained approximately equal amounts of Hsp27 and Hsp20 subunits. Phosphorylation of Hsp27 by mitogen activated protein kinase-activated .

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