TAILIEUCHUNG - Báo cáo khoa học: Purine-induced expression of urate oxidase and enzyme activity in Atlantic salmon (Salmo salar)

The peroxisomal enzyme urate oxidase plays a pivotal role in the degrada-tion of purines in both prokaryotes and eukaryotes. However, knowledge about the purine-induced expression of the encoding gene is lacking in vertebrates. These are the first published sequences of fish urate oxidase, | ềFEBS Journal Purine-induced expression of urate oxidase and enzyme activity in Atlantic salmon Salmo salar Cloning of urate oxidase liver cDNA from three teleost species and the African lungfish Protopterus annectens 0ivind Andersen1 2 Turid S. Aas1 Stanko Skugor1 Harald Takle1 Solveig van Nes1 Barbara Grisdale-Helland1 2 Stille J. Helland1 2 and Bendik F. Terjesen1 2 1 AKVAFORSK Institute of Aquaculture Research AS As Norway 2 Aquaculture Protein Centre CoE Norway Keywords African lungfish Atlantic salmon urate urate oxidase uricase Correspondence 0. Andersen AKVAFORSK PO Box 5010 N-1432 As Norway Tel 47 64965261 E-mail Received 11 January 2006 revised 7 April 2006 accepted 19 April 2006 doi The peroxisomal enzyme urate oxidase plays a pivotal role in the degradation of purines in both prokaryotes and eukaryotes. However knowledge about the purine-induced expression of the encoding gene is lacking in vertebrates. These are the first published sequences of fish urate oxidase which were predicted from PCR amplified liver cDNAs of Atlantic salmon Salmo salar Atlantic cod Gadus morhua Atlantic halibut Hippoglossus hippoglossus and African lungfish Protopterus annectens . Sequence alignment of different vertebrate urate oxidases revealed amino acid substitutions of putative functional importance in the enzyme of chicken and lungfish. In the adult salmon expression of urate oxidase mRNA predominated in liver but was also identified in several nonhepatic organs including brain but not in skeletal muscle and kidney. Juvenile salmon fed diets containing bacterial protein meal BPM rich in nucleic acids showed a significant increase in liver urate oxidase enzyme activity and urea concentrations in plasma muscle and liver were elevated. Whereas salmon fed the 18 BPM diet showed a nonsignificant increase in liver mRNA levels of urate oxidase compared with the 0 BPM-fed fish no further increase in mRNA levels was .

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