TAILIEUCHUNG - Báo cáo khoa học: Cell-free translation systems for protein engineering

Cell-free translation systems have developed significantly over the last two decades and improvements in yield have resulted in their use for protein production in the laboratory. These systems have protein engineering appli-cations, such as the production of proteins containing unnatural amino acids and development of proteins exhibiting novel functions. | ỊFEBS Journal MINIREVIEW Cell-free translation systems for protein engineering Yoshihiro Shimizu1 Yutetsu Kuruma2 Bei-Wen Ying1 So Umekage3 and Takuya Ueda1 1 Department of MedicalGenome Sciences Graduate Schoolof Frontier Sciences The University of Tokyo Kashiwanoha Kashiwa-shi Chiba Japan 2 Enrico Fermi Center Compendio delViminale Rome Italy 3 Division of Bioscience and Biotechnology Department of EcologicalEngineering Toyohashi University of Technology Tempaku-cho Toyohashi Aichi Japan Keywords cell-free protein synthesis chaperone disulfide bond formation in vitro selection liposome minimal cell ribosome display translation unnatural amino acid Correspondence T. Ueda Department of MedicalGenome Sciences Graduate School of Frontier Sciences University of Tokyo FSB401 5-1-5 Kashiwanoha Kashiwa-shi Chiba prefecture 277-8562 Japan Fax 81 4 7136 3642 Tel 81 4 7136 3641 E-mail ueda@ Received 8 May 2006 revised 20 June 2006 accepted 26 June 2006 doi Cell-free translation systems have developed significantly over the last two decades and improvements in yield have resulted in their use for protein production in the laboratory. These systems have protein engineering applications such as the production of proteins containing unnatural amino acids and development of proteins exhibiting novel functions. Recently it has been suggested that cell-free translation systems might be used as the fundamental basis for cell-like systems. We review recent progress in the field of cell-free translation systems and describe their use as tools for protein production and engineering. Introduction Although noncoding RNAs play significant roles in cellular function 1 2 especially in higher organisms it is proteins that dominate most cellular processes. Proteins are the most abundant cellular components and are responsible for structural metabolic and regulatory functions both inside and outside of cells. Thus investigation of proteins and .

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