TAILIEUCHUNG - Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera

The vertex of the adenoviral capsid is formed by the penton, a complex of two proteins, the pentameric penton base and the trimeric fiber protein. The penton contains all necessary components for viral attachment and entry into the host cell. After initial attachment via the head domain of the fiber protein, the penton base interacts with cellular integrins through an Arg-Gly-Asp (RGD) motif located in a hypervariable surface loop, trigger-ing virus internalization. | ễFEBS Journal Structural and biochemical characterization of a human adenovirus 2 12 penton base chimera Chloe Zubieta1 Laurent Blanchoin2 and Stephen Cusack1 1 European Molecular Biology Laboratory Grenoble Outstation France 2 Laboratoire de Physiologie Cellulaire Vegetale Commissariat a l Energie Atomique Centre Nationalde la Recherche Scientifique Institut Nationalde la Recherche Agronomique Universite Joseph Fourier Unite Mixte de Recherche 5168 Grenoble France Keywords adenovirus crystal structure fiber fluorescence anisotropy penton Correspondence C. Zubieta SLAC SSRL 2575 Sandhill Road Menlo Park CA 94025 USA Fax 1 650 926 3292 Tel 1 650 926 2992 E-mail czubieta@ Present address Stanford Synchrotron Radiation Facility Menlo Park CA USA Received 6 June 2006 revised 11 July 2006 accepted 27 July 2006 doi The vertex of the adenoviral capsid is formed by the penton a complex of two proteins the pentameric penton base and the trimeric fiber protein. The penton contains all necessary components for viral attachment and entry into the host cell. After initial attachment via the head domain of the fiber protein the penton base interacts with cellular integrins through an Arg-Gly-Asp RGD motif located in a hypervariable surface loop triggering virus internalization. In order to investigate the structural and functional role of this region we replaced the hypervariable loop of serotype 2 with the corresponding but much shorter loop of serotype 12 and compared it to the wild type. Here we report the A crystal structure of a human adenovirus 2 12 penton base chimera crystallized as a dodecamer. The structure is generally similar to human adenovirus 2 penton base with the main differences localized to the fiber protein-binding site. Fluorescence anisotropy assays using a trimeric fiber protein mimetic called the minifiber and wild-type human adenovirus 2 and chimeric penton base demonstrate that fiber protein binding

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical research
• hemoglobin
• medical knowledge
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine